CD Antigens
Product List
CD23 Human, Sf9
CD23 Human Recombinant, Sf9
CD23 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 283 amino acids (48-321a.a.) and having a molecular mass of 32.0kDa.
CD23 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
sCD23 Human
Soluble CD23 Human Recombinant
Introduction
CD23, also known as Fc epsilon RII (FcεRII), is a low-affinity receptor for Immunoglobulin E (IgE), an antibody isotype involved in allergic reactions and resistance to parasites . CD23 is a C-type lectin and is classified into two forms: CD23a and CD23b. CD23a is present on follicular B cells, while CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages .
Key Biological Properties: CD23 is a type II transmembrane glycoprotein with a molecular weight of approximately 45 kDa . It consists of a large C-terminal globular extracellular domain, a stalk region, a single hydrophobic membrane-spanning region, and a short N-terminal cytoplasmic domain .
Expression Patterns and Tissue Distribution: CD23 is expressed on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets . It is also found in various tissues, including the spleen, lymph nodes, and bone marrow .
Primary Biological Functions: CD23 plays a crucial role in the regulation of IgE levels and the transportation of IgE immune complexes . It is involved in antibody feedback regulation, where antigens captured by IgE antibodies bind to CD23 molecules on B cells and are transported to the B cell follicles of the spleen .
Role in Immune Responses and Pathogen Recognition: CD23 is essential in modulating immune responses, particularly in allergic reactions. It helps regulate the synthesis of IgE, the antibody isotype that mediates allergic responses .
Mechanisms with Other Molecules and Cells: CD23 interacts with IgE and CD21, regulating the synthesis of IgE . It can be cleaved from cell surfaces to yield soluble CD23 (sCD23) proteins that have cytokine-like activities .
Binding Partners and Downstream Signaling Cascades: CD23 binds to IgE and CD21, influencing various immune pathways. The activation of CD23 can result in the downregulation of IgE production or modulation of B cell activity, reducing inflammation and allergic responses .
Regulatory Mechanisms Controlling Expression and Activity: CD23 expression is regulated by several cytokines, including interleukin-4 (IL-4) and interferons . It can be cleaved from cell surfaces to yield soluble CD23 proteins, which have pleiotropic cytokine-like activities .
Transcriptional Regulation and Post-Translational Modifications: The gene encoding CD23 is located on chromosome 19 and is regulated by various stimuli . Post-translational modifications, such as glycosylation, play a role in its function and stability .
Biomedical Research: CD23 is used as a marker in the diagnosis of chronic lymphocytic leukemia (CLL) and other lymphoid leukemias . It is also a target for therapeutic intervention in allergic diseases and autoimmune conditions .
Diagnostic Tools and Therapeutic Strategies: CD23 agonists are being explored for their potential in treating allergic diseases such as asthma, allergic rhinitis, and atopic dermatitis . These agonists can modulate immune responses by targeting CD23, providing relief from symptoms and improving the quality of life for patients .
Role Throughout the Life Cycle: CD23 plays a role in the development and growth of B cells. It is involved in the regulation of IgE levels and immune responses throughout the life cycle . CD23’s function in antibody feedback regulation and immune modulation is crucial from development to aging and disease .
