CD23 Human, Sf9
Sf9, Baculovirus cells.
Low affinity immunoglobulin epsilon Fc receptor isoform a, BLAST-2, CD23, CD23A, CLEC4J, FCE2, IGEBF, C-type lectin domain family 4 member J, Immunoglobulin E-binding factor, Lymphocyte IgE receptor.
Greater than 90.0% as determined by SDS-PAGE.
Description
CD23 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 283 amino acids (48-321a.a.) and having a molecular mass of 32.0kDa.
CD23 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
FCER2, also known as Fc epsilon RII, is a low-affinity receptor for IgE and plays a crucial role in regulating IgE levels. IgE, an antibody isotype, is associated with allergic responses and defense against parasites. Unlike many antibody receptors, FCER2 belongs to the C-type lectin family. It is found on the surface of activated macrophages, mature B cells, follicular dendritic cells, platelets, and eosinophils.
Recombinant human CD23, expressed in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain. It consists of 283 amino acids (residues 48-321), resulting in a molecular weight of 32.0 kDa. The protein contains a 6-amino acid Histidine tag at the C-terminus to facilitate purification, which is performed using proprietary chromatographic techniques.
The CD23 protein is supplied as a solution at a concentration of 0.25 mg/ml in phosphate-buffered saline (pH 7.4) containing 10% glycerol.
The purity of CD23 protein is determined to be greater than 90% by SDS-PAGE analysis.
Low affinity immunoglobulin epsilon Fc receptor isoform a, BLAST-2, CD23, CD23A, CLEC4J, FCE2, IGEBF, C-type lectin domain family 4 member J, Immunoglobulin E-binding factor, Lymphocyte IgE receptor.
Sf9, Baculovirus cells.
ADPDTTQSLK QLEERAARNV SQVSKNLESH HGDQMAQKSQ STQISQELEE LRAEQQRLKS QDLELSWNLN GLQADLSSFK SQELNERNEA SDLLERLREE VTKLRMELQV SSGFVCNTCP EKWINFQRKC YYFGKGTKQW VHARYACDDM EGQLVSIHSP EEQDFLTKHA SHTGSWIGLR
NLDLKGEFIW VDGSHVDYSN WAPGEPTSRS QGEDCVMMRG SGRWNDAFCD RKLGAWVCDR LATCTPPASE GSAESMGPDS RPDPDGRLPT
PSAPLHSHHH HHH.
Product Science Overview
CD23 is a type II transmembrane protein that belongs to the C-type lectin family. It has a single carbohydrate recognition domain (CRD) at its extracellular C-terminus, which is responsible for binding to IgE. The binding of IgE to CD23 is essential for the regulation of IgE levels in the body and plays a significant role in allergic reactions .
The recombinant expression of CD23 in Sf9 cells (a cell line derived from the fall armyworm, Spodoptera frugiperda) is a common method used to produce large quantities of the protein for research purposes. Sf9 cells are often used in the baculovirus expression system, which allows for high-level expression of recombinant proteins. This system is particularly advantageous for producing proteins that require post-translational modifications, such as glycosylation, which are essential for the proper folding and function of CD23 .
Recombinant CD23 produced in Sf9 cells has been extensively used in various research studies to understand its structure, function, and role in the immune system. For instance, studies have shown that CD23 can form a trimolecular complex with IgE and allergens, which is crucial for the presentation of allergens to T cells and the subsequent activation of allergen-specific T cells . This interaction is important for the development of allergic responses and has been a target for therapeutic interventions aimed at treating allergies.
Additionally, CD23 has been implicated in the differentiation of B cells into plasmablasts, which are antibody-secreting cells. The downregulation of CD23 and the IL-4 signaling pathway is a critical step in this differentiation process . Understanding these mechanisms is essential for developing new therapeutic strategies for immune-related disorders and lymphoproliferative diseases.
