CD Antigens
Product List
CD247 Human
CD247 Human Recombinant
CD247 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CD247 Human, Sf9
CD247 Human Recombinant, Sf9
CD247 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 122 amino acids (52-164a.a.) and having a molecular mass of 14.1kDa. (Molecular size on SDS-PAGE will appear at approximately 13.5-18kDa). CD247 is expressed with an 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Introduction
CD247, also known as the T-cell surface glycoprotein CD3 zeta chain, is a protein encoded by the CD247 gene in humans. It is a crucial component of the T-cell receptor (TCR) complex, which plays a vital role in the adaptive immune response . CD247 is classified as a protein-coding gene and is also referred to by several other names, including CD3Z, CD3H, CD3Q, and TCRZ .
Key Biological Properties: CD247 is a 16-kDa transmembrane protein primarily expressed in T cells and natural killer (NK) cells . It contains three immunoreceptor tyrosine-based activation motifs (ITAMs) in its intracellular domain, which are essential for signal transduction .
Expression Patterns and Tissue Distribution: CD247 is predominantly expressed in lymphoid tissues such as the thymus, spleen, and lymph nodes . It is also found in other tissues, including the bone marrow, testis, and skeletal muscle .
Primary Biological Functions: CD247 is a critical component of the TCR-CD3 complex, which is essential for T-cell activation and signaling . It plays a pivotal role in coupling antigen recognition to intracellular signal transduction pathways, leading to T-cell proliferation, differentiation, and cytokine production .
Role in Immune Responses and Pathogen Recognition: CD247 is involved in the recognition of antigens presented by antigen-presenting cells (APCs) and the subsequent activation of T cells . This activation is crucial for mounting an effective immune response against pathogens .
Mechanisms with Other Molecules and Cells: CD247 interacts with other components of the TCR-CD3 complex, including CD3-gamma, -delta, and -epsilon chains . Upon antigen recognition, the ITAMs in CD247 become phosphorylated by Src family kinases such as LCK and FYN .
Binding Partners and Downstream Signaling Cascades: The phosphorylation of ITAMs creates docking sites for downstream signaling molecules, including ZAP-70, which further propagates the signal through various pathways such as the Ras-MEK-ERK pathway and the activation of transcription factors like NF-kB, NFAT, and AP-1 .
Regulatory Mechanisms Controlling Expression and Activity: The expression of CD247 is regulated at both the transcriptional and post-transcriptional levels . Transcription factors such as NFAT and AP-1 play a role in regulating CD247 gene expression .
Transcriptional Regulation and Post-Translational Modifications: Post-translational modifications, including phosphorylation of ITAMs, are critical for the activation and function of CD247 . These modifications are essential for the proper assembly and signaling of the TCR-CD3 complex .
Biomedical Research: CD247 is widely used as a marker in immunological studies to investigate T-cell activation and function .
Diagnostic Tools: CD247 expression levels can serve as a diagnostic biomarker for various diseases, including immunodeficiencies and chronic inflammatory conditions .
Therapeutic Strategies: Targeting CD247 and its signaling pathways holds potential for developing therapeutic strategies for autoimmune diseases, cancers, and other immune-related disorders .
Role Throughout the Life Cycle: CD247 plays a crucial role in the development and maturation of T cells in the thymus . Its expression and function are essential for maintaining immune homeostasis throughout life .
From Development to Aging and Disease: Alterations in CD247 expression or function can lead to various immune-related diseases, including immunodeficiencies and autoimmune disorders . Understanding the role of CD247 in these processes is vital for developing targeted therapies .
