CD247 Human
Description
CD247 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The CD247 gene encodes the T-cell receptor zeta chain, which is a part of the CD3 complex. The CD3 complex is composed of several chains: CD3-gamma, CD3-delta, CD3-epsilon, and CD3-zeta. The zeta chain, encoded by CD247, is essential for the proper functioning of the TCR complex .
The CD247 protein contains three Immunoreceptor Tyrosine-based Activation Motifs (ITAMs), which are critical for signal transduction. These motifs allow the zeta chain to couple antigen recognition to several intracellular signaling pathways .
The primary function of CD247 is to form the TCR-CD3 complex along with T-cell receptor alpha/beta and gamma/delta heterodimers. This complex is vital for the immune response, as it enables T-cells to recognize and respond to antigens. The zeta chain, in particular, plays a pivotal role in coupling antigen recognition to intracellular signal transduction pathways .
Low expression or defects in CD247 can lead to impaired immune responses. For instance, mutations in the CD247 gene can cause immunodeficiency due to defects in the CD3-zeta chain. This condition is characterized by T-cells’ impaired response to alloantigens, tetanus toxoid, and mitogens .
Recombinant CD247 is produced using various expression systems, such as E. coli. The recombinant protein is often tagged with GST (Glutathione S-transferase) to facilitate purification and detection. The recombinant CD247 protein is used in research to study T-cell activation, signal transduction, and immune responses .
Recombinant CD247 is widely used in immunological research. It helps in understanding the mechanisms of T-cell activation and the role of the TCR-CD3 complex in immune responses. Additionally, it is used to study the effects of mutations in the CD247 gene and to develop potential therapeutic interventions for immunodeficiency disorders .
Recombinant CD247 is typically provided as a lyophilized powder and should be stored at -20°C to -80°C for long-term stability. Once reconstituted, the protein solution can be stored at 4-8°C for short-term use. It is essential to handle the protein under sterile conditions to prevent contamination .
