CD247 Human, Sf9
CD247 Molecule, T-Cell Surface Glycoprotein CD3 Zeta Chain, T-Cell Receptor T3 Zeta Chain, CD247 Antigen, CD3Z, TCRZ, T3Z, T-Cell Antigen Receptor Complex, Zeta Subunit Of CD3, CD3Z Antigen, Zeta Polypeptide (TiT3 Complex), CD3z Antigen, Zeta Polypeptide (TiT3 Complex) , CD247 Antigen, Zeta Subunit , TCR Zeta Chain, CD3zeta Chain, CD3-ZETA, IMD25, CD3H, CD3Q.
Greater than 90.0% as determined by SDS-PAGE.
Description
CD247 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 122 amino acids (52-164a.a.) and having a molecular mass of 14.1kDa. (Molecular size on SDS-PAGE will appear at approximately 13.5-18kDa). CD247 is expressed with an 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD247 Molecule, T-Cell Surface Glycoprotein CD3 Zeta Chain, T-Cell Receptor T3 Zeta Chain, CD247 Antigen, CD3Z, TCRZ, T3Z, T-Cell Antigen Receptor Complex, Zeta Subunit Of CD3, CD3Z Antigen, Zeta Polypeptide (TiT3 Complex), CD3z Antigen, Zeta Polypeptide (TiT3 Complex) , CD247 Antigen, Zeta Subunit , TCR Zeta Chain, CD3zeta Chain, CD3-ZETA, IMD25, CD3H, CD3Q.
ADPRVKFSRS ADAPAYQQGQ NQLYNELNLG RREEYDVLDK RRGRDPEMGG KPQRRKNPQE GLYNELQKDK MAEAYSEIGM KGERRRGKGH DGLYQGLSTA TKDTYDALHM QALPPRHHHH HH
Product Science Overview
CD247 is a component of the T-cell receptor (TCR) complex, which is essential for the recognition of antigens presented by the major histocompatibility complex (MHC) on antigen-presenting cells. The TCR complex is composed of several subunits, including the CD3 epsilon, delta, gamma, and zeta chains. The CD3 zeta chain (CD247) is particularly important for transmitting activation signals from the TCR to the cell’s interior, leading to T-cell activation and subsequent immune responses.
Recombinant human CD247 is produced using Sf9 insect cells through a process known as the baculovirus expression system. This system is widely used for the production of recombinant proteins due to its ability to produce high yields of properly folded and post-translationally modified proteins.
The recombinant CD247 expressed in Sf9 cells is typically a single, glycosylated polypeptide chain containing 122 amino acids (52-164 a.a.) and has a molecular weight of approximately 14.1 kDa . On SDS-PAGE, the molecular size of recombinant CD247 appears at approximately 13.5-18 kDa .
Recombinant CD247 has several applications in research and clinical settings, including:
- Immunological Studies: Understanding the role of CD247 in T-cell activation and signal transduction.
- Drug Development: Screening for potential therapeutic agents that target the TCR complex.
- Diagnostics: Developing assays for detecting T-cell-related disorders.
