CD200 Human, Sf9
CD200, MOX1, MOX2, MRC, OX-2, CD200 Molecule, CD200 Antigen, Antigen Identified By Monoclonal Antibody MRC OX-2, OX-2 Membrane Glycoprotein.
Greater than 95.0% as determined by SDS-PAGE.
Description
CD200 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 444 amino acids (31-232a.a.) and having a molecular mass of 49.7kDa (Molecular size on SDS-PAGE will appear at approximately 57-70 kDa).
CD200 is expressed with a 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD200, a member of the immunoglobulin superfamily, is a type-1 membrane glycoprotein characterized by its two immunoglobulin domains. It plays a crucial role in regulating myeloid cell activity, particularly by delivering inhibitory signals to macrophages across various tissues. Moreover, CD200 exhibits costimulatory effects on T-cell proliferation.
Recombinant Human CD200, expressed in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It consists of 444 amino acids (31-232a.a.), resulting in a molecular mass of 49.7kDa. Notably, the molecular size on SDS-PAGE analysis is expected to appear between 57-70 kDa. The protein is engineered with a 242 amino acid hIgG-His tag at the C-terminus and purified using proprietary chromatographic techniques.
The CD200 protein solution is provided at a concentration of 0.5mg/ml. It is formulated in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
The purity of the CD200 protein is determined to be greater than 95.0% as assessed by SDS-PAGE analysis.
CD200, MOX1, MOX2, MRC, OX-2, CD200 Molecule, CD200 Antigen, Antigen Identified By Monoclonal Antibody MRC OX-2, OX-2 Membrane Glycoprotein.
ADPQVQVVTQ DEREQLYTPA SLKCSLQNAQ EALIVTWQKK KAVSPENMVT FSENHGVVIQ PAYKDKINIT QLGLQNSTIT FWNITLEDEG CYMCLFNTFG FGKISGTACL TVYVQPIVSL HYKFSEDHLN ITCSATARPA PMVFWKVPRS GIENSTVTLS HPNGTTSVTS ILHIKDPKNQ VGKEVICQVL HLGTVTDFKQ TVNKGLEPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH.
Product Science Overview
CD200 is a single, glycosylated polypeptide chain. The recombinant form of CD200 produced in Sf9 Baculovirus cells consists of 444 amino acids and has a molecular mass of approximately 49.7 kDa . The protein is expressed with a 242 amino acid hIgG-His tag at the C-terminus and is purified using proprietary chromatographic techniques .
CD200 is involved in the regulation of myeloid cell activity and delivers an inhibitory signal to the macrophage lineage in various tissues . This interaction is essential for preventing excessive macrophage activation, which can lead to tissue damage and inflammation. Additionally, CD200 costimulates T-cell proliferation, further highlighting its role in immune modulation .
Dysfunction or altered expression of CD200 has been associated with several diseases, including Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy . The study of CD200 and its interactions with CD200R is crucial for understanding immune regulation and developing potential therapeutic interventions for immune-related disorders.
Recombinant CD200 proteins, such as those produced in Sf9 cells, are widely used in research to study immune regulation and to develop therapeutic strategies. These proteins are often used in ELISA assays and other biochemical applications to investigate the binding interactions between CD200 and CD200R .
