CD200R1 Human
Sf9, Baculovirus cells.
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Cell surface glycoprotein CD200 receptor 1 isoform d, CD200R1, CD200R, HCRTR2, MOX2R, OX2R, Cell surface glycoprotein CD200 receptor 1, CD200 cell surface glycoprotein receptor, Cell surface glycoprotein OX2 receptor 1, Cd200r1, Mox2r, Ox2r. |
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
CD200R1 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 462 amino acids (24-243 a.a.) and having a molecular mass of 51.9kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). CD200R1 is expressed with 242 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
CD200R1, alternatively known as cell surface glycoprotein CD200 receptor 1, is an isoform of CD200 receptors primarily found on myeloid lineage cells. This receptor's inhibitory signaling pathway plays a crucial role in regulating inflammatory responses across a wide array of inflammatory conditions. Specifically, CD200R1 signaling effectively suppresses the production of pro-inflammatory mediators such as tumor necrosis factor, interferons, and inducible nitric oxide synthase in response to specific stimuli.
Produced in Sf9 Insect cells, CD200R1 is a single, glycosylated polypeptide chain consisting of 462 amino acids (specifically, amino acids 24-243). It possesses a molecular mass of 51.9kDa, although its apparent size on SDS-PAGE may range from approximately 50-70kDa. This protein is expressed with a 242 amino acid His tag at the C-terminus and undergoes purification using proprietary chromatographic techniques.
A clear, colorless solution that has been sterilized by filtration.
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The provided CD200R1 protein solution has a concentration of 0.25mg/ml and is formulated in a solution of phosphate-buffered saline (pH 7.4) containing 10% glycerol. |
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (either HSA or BSA) to a final concentration of 0.1% is advisable for long-term storage. To maintain product integrity, avoid repeated cycles of freezing and thawing.
Purity levels exceed 90.0% as determined by SDS-PAGE analysis.
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Cell surface glycoprotein CD200 receptor 1 isoform d, CD200R1, CD200R, HCRTR2, MOX2R, OX2R, Cell surface glycoprotein CD200 receptor 1, CD200 cell surface glycoprotein receptor, Cell surface glycoprotein OX2 receptor 1, Cd200r1, Mox2r, Ox2r. |
Sf9, Baculovirus cells.
ADLSSSLCMD EKQITQNYSK VLAEVNTSWP VKMATNAVLC CPPIALRNLI IITWEIILRG QPSCTKAYKK ETNETKETNC TDERITWVSR PDQNSDLQIR TVAITHDGYY RCIMVTPDGN FHRGYHLQVL VTPEVTLFQN RNRTAVCKAV AGKPAAHISW IPEGDCATKQ EYWSNGTVTV KSTCHWEVHN VSTVTCHVSH LTGNKSLYIE LLPVPGAKKS AKLLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH.
Product Science Overview
CD200R1 contains:
- One C2-type immunoglobulin-like domain
- One V-type immunoglobulin-like domain within its extracellular domain
- A PTB-signaling motif in its cytoplasmic domain
The receptor and its ligand, CD200, associate via their respective N-terminal immunoglobulin-like domains . CD200R1 is primarily expressed on the surfaces of myeloid lineage cells, including mast cells, basophils, macrophages, and dendritic cells .
CD200R1 is involved in propagating inhibitory signals, despite lacking a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) . The receptor-substrate interaction functions as a myeloid downregulatory signal, which is essential for maintaining immune homeostasis and preventing overactivation of myeloid cells .
