Cytokines
Product List
EBI3 Human
Epstein Barr Virus Induced 3 Human Recombinant
The EBI3 is purified by proprietary chromatographic techniques.
EBI3 Human, His
Epstein Barr Virus Induced 3 Human Recombinant, His Tag
The EBI3 is purified by proprietary chromatographic techniques.
EBI3 Macaque
Epstein Barr Virus Induced 3 Macaque Recombinant
EBI3 Macaque Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 210 amino acids and having a molecular mass of 23.4kDa. The EBI3 is purified by proprietary chromatographic techniques.
Escherichia Coli.
EBI3 Mouse
Epstein Barr Virus Induced 3 Mouse Recombinant
The Murine EBI3 is purified by proprietary chromatographic techniques.
Introduction
EBI3, or Epstein-Barr virus-induced gene 3, is a protein encoded by the EBI3 gene in humans. It is also known as interleukin-27 subunit beta (IL-27B) and interleukin-35 subunit beta (IL-35B). EBI3 is a member of the hematopoietin receptor family and is related to the p40 subunit of interleukin-12 (IL-12). It forms part of two distinct heterodimeric cytokines: interleukin-27 (IL-27) and interleukin-35 (IL-35) .
EBI3 is a secreted glycoprotein that plays a crucial role in immune responses. It is expressed in various tissues, including the placenta, spleen, lymph nodes, and appendix. The expression of EBI3 is induced in B lymphocytes by Epstein-Barr virus infection and is also regulated by Toll-like receptor (TLR) signaling in dendritic cells .
EBI3 is involved in regulating cell-mediated immune responses. It forms part of IL-27 and IL-35, which have distinct roles in the immune system. IL-27, composed of EBI3 and p28, can trigger signaling in T cells, B cells, and myeloid cells, promoting T-helper cell development and suppressing T-cell proliferation. IL-35, composed of EBI3 and p35, is involved in regulatory T-cell function and has inhibitory effects on immune responses .
EBI3 interacts with other molecules and cells through its role in forming IL-27 and IL-35. IL-27 binds to the cytokine receptor WSX-1/TCCR, triggering downstream signaling cascades that involve the JAK/STAT pathway. IL-35 also signals through the JAK/STAT pathway, modulating the activity of regulatory T cells and suppressing inflammatory responses. Additionally, EBI3 can bind to cytokines such as IFN-γ and IL-10, acting as a cytokine sink and limiting their functions .
The expression and activity of EBI3 are regulated at multiple levels. Transcriptional regulation involves NF-κB and Ets binding sites, which are activated by TLR signaling. Post-translational modifications, such as glycosylation, also play a role in the stability and function of EBI3. Furthermore, EBI3 collaborates with the chaperone protein calnexin to promote the proper folding and expression of target proteins under inflammatory conditions .
EBI3 has several applications in biomedical research, diagnostic tools, and therapeutic strategies. It is used in studies investigating immune responses and inflammatory diseases. EBI3 is also a target for developing diagnostic assays for conditions such as autoimmune diseases and certain cancers. Therapeutically, modulating EBI3 activity has potential in treating inflammatory and immune-related disorders .
EBI3 plays a role throughout the life cycle, from development to aging and disease. During development, EBI3 is involved in the regulation of immune responses and the proper folding of cytokines. In aging, EBI3 expression can be altered, contributing to changes in immune function. In disease, dysregulation of EBI3 is associated with conditions such as autoimmune diseases, cancers, and chronic inflammatory disorders .
