EBI3 Human, His
Epstein Barr Virus Induced 3 Human Recombinant, His Tag
Cat. No.
BT20859
Source
Escherichia Coli.
Synonyms
Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI3, IL27B.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
EBI3 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 209 amino acids fragment (21-229) having a molecular weight of 34kDa and fused with a 4.5kDa amino-terminal hexahistidine tag.
The EBI3 is purified by proprietary chromatographic techniques.
The EBI3 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
When B lymphocytes are infected with the Epstein-Barr virus, the expression of EBI3 is induced. EBI3 encodes a secreted glycoprotein that belongs to the hematopoietin receptor family. It forms iIL-27 by heterodimerizing with a 28 kDa protein. EBI3 drives rapid clonal expansion of naive cd4(+) t-cells and strongly synergizes with IL-12 to activate IFN-gamma production of naive cd4(+) t-cells. EBI3's biological effects are mediated through the cytokine receptor wsx-1/tccr.
Description
Recombinant human EBI3 is produced in E. coli. It is a single, non-glycosylated polypeptide chain containing amino acids 21-229 of EBI3 and a 4.5kDa amino-terminal hexahistidine tag. This results in a protein fragment with a molecular weight of 34kDa. EBI3 is purified using proprietary chromatographic techniques.
Physical Appearance
The product is a clear solution that has been sterilized by filtration.
Formulation
EBI3 protein is supplied in a solution of 1xPBS and 50% glycerol.
Stability
If the entire vial will be used within 2-4 weeks, the product can be stored at 4°C. For longer storage periods, the product should be stored frozen at -20°C. Repeated freezing and thawing should be avoided.
Purity
Purity is determined by SDS-PAGE to be greater than 95.0%.
Synonyms
Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI3, IL27B.
Source
Escherichia Coli.
Product Science Overview
Role in the Immune System
EBI3 is a subunit of both interleukin (IL)-27 and IL-35 . These interleukins play crucial roles in the immune system:
- IL-27: Involved in the regulation of T-helper cell development and has both pro- and anti-inflammatory properties.
- IL-35: Known for its immunosuppressive functions, helping to regulate immune responses and maintain immune tolerance.
Recombinant EBI3
The recombinant form of EBI3, tagged with a His (histidine) tag, is used in various research applications. The His tag facilitates the purification and detection of the protein in experimental settings . This recombinant protein is valuable for studying the biological functions of EBI3 and its role in immune responses.
Applications in Research
Recombinant EBI3 is used in:
- Immunological studies: To understand its role in immune regulation and its potential therapeutic applications.
- Protein interaction studies: To investigate how EBI3 interacts with other proteins, such as its partners IL-27 p28 and IL-35 p35 .
- Disease research: To explore its involvement in diseases where the immune system plays a critical role, such as autoimmune diseases and cancers.
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