EBI3 Mouse
Epstein Barr Virus Induced 3 Mouse Recombinant
Cat. No.
BT21556
Source
Escherichia Coli.
Synonyms
IL-27B, IL27B, IL 27-B, EBI-3, Interleukin-27 beta, IL-27 subunit beta, Epstein-Barr virus-induced gene 3 protein homolog, EBI3.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
EBI3 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 22.9kDa.
The Murine EBI3 is purified by proprietary chromatographic techniques.
The Murine EBI3 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
When B lymphocytes are infected with the Epstein-Barr virus, the expression of EBI3 is induced. This gene codes for a secreted glycoprotein, which is part of the hematopoietin receptor family. EBI3 forms a heterodimer with a 28 kDa protein to create iIL-27. EBI3 causes naive cd4(+) t-cells to undergo rapid clonal expansion, and it works with IL-12 to strongly activate IFN-gamma production in these cells. It carries out its biological functions via the cytokine receptor wsx-1/tccr.
Description
Recombinant Mouse EBI3, produced in E. coli, is a single, non-glycosylated polypeptide chain. This protein comprises 207 amino acids, resulting in a molecular mass of 22.9 kDa. The purification of Murine EBI3 is achieved through proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
For the purpose of lyophilization, EBI3 was prepared in a solution of 10mM Sodium Citrate with a pH of 3.
Solubility
To reconstitute the lyophilized EBI3, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration not less than 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized EBI3 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated below -18°C. Upon reconstitution, EBI3 should be stored at 4°C for a period of 2-7 days. For future use, it should be stored below -18°C. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 90% by (a) Analysis using RP-HPLC. (b) Analysis using SDS-PAGE.
Synonyms
IL-27B, IL27B, IL 27-B, EBI-3, Interleukin-27 beta, IL-27 subunit beta, Epstein-Barr virus-induced gene 3 protein homolog, EBI3.
Source
Escherichia Coli.
Amino Acid Sequence
MALVALSQPR VQCHASRYPV AVDCSWTPLQ APNSTRSTSF IATYRLGVAT QQQSQPCLQR SPQASRCTIP DVHLFSTVPY MLNVTAVHPG GASSSLLAFV AERIIKPDPP EGVRLRTAGQ RLQVLWHPPA SWPFPDIFSL KYRLRYRRRG ASHFRQVGPI EATTFTLRNS KPHAKYCIQV SAQDLTDYGK PSDWSLPGQV ESAPHKP.
Product Science Overview
Structure and Function
Biological Role
EBI3 plays a crucial role in the immune response. It is involved in the regulation of T-cell responses and has been shown to have both pro-inflammatory and anti-inflammatory effects depending on the context . The protein is particularly important in the context of viral infections, such as those caused by EBV, where it helps modulate the immune response to prevent excessive inflammation .
Recombinant Mouse EBI3
Recombinant Mouse EBI3 is produced using E. coli expression systems and is used extensively in research to study its function and potential therapeutic applications . The recombinant protein is typically purified to a high degree of purity (>90%) and is used in various assays to understand its binding properties and biological activities .
Applications in Research
Recombinant Mouse EBI3 is used in a variety of research applications, including:
- Functional assays: To study the binding and activity of EBI3 in different biological contexts .
- Immunological studies: To understand the role of EBI3 in immune regulation and its potential as a therapeutic target .
- Protein interaction studies: To investigate how EBI3 interacts with other proteins and cytokines .
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