EBI3 Macaque

EBI3 forms heterodimers with other proteins to create interleukins with significant biological functions:

• Interleukin 27 (IL-27): Formed by EBI3 and p28, IL-27 has anti-inflammatory and regulatory activity .
• Interleukin 35 (IL-35): Formed by EBI3 and p35, IL-35 also exhibits anti-inflammatory properties .

The recombinant form of Macaque Epstein-Barr Virus Induced Gene-3 is a non-glycosylated protein monomer, containing 210 amino acids and having a molecular mass of 23.4 kDa . This recombinant protein is typically produced in E. coli and is used for research purposes .

Recombinant EBI3 is used in various research applications, particularly in studies related to immune response and inflammation. The lyophilized product is stable at -20°C, and once reconstituted, it should be aliquoted and stored at -20°C with a carrier protein for long-term stability .

To reconstitute the lyophilized product, it is recommended to use sterile 20 mM HCl at a concentration of 0.1 mg/mL, which can then be further diluted into other aqueous solutions . The protein content and purity are determined by UV spectroscopy at 280 nm, RP-HPLC calibrated against a known standard, and quantitation against a known standard via reducing and non-reducing SDS-PAGE gels . The endotoxin level is measured to be less than 0.01 ng/µg or less than 0.1 EU/µg .

EBI3 plays a crucial role in the immune system by forming interleukins that regulate inflammation and immune responses. Its recombinant form allows researchers to study its functions and interactions in a controlled environment, contributing to our understanding of immune mechanisms and potential therapeutic targets.