Cytokines
Product List
LIF Human
Leukemia Inhibitory Factor Human Recombinant
The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques.
LIF Human, GST
Leukemia Inhibitory Factor, GST tag Human Recombinant
LIF is fused to a 236 amino acid His-GST tag at N-terminus & purified by proprietary chromatographic techniques.
LIF Human, His
Leukemia Inhibitory Factor Human Recombinant, His tag
LIF Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 23-202) containing 189 amino acids including a 9 a.a N-terminal His tag. The total molecular mass is 20.9kDa (calculated).
Escherichia Coli.
LIF Human, Sf9
Leukemia Inhibitory Factor Human Recombinant, Sf9
LIF Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 189 amino acids (23-202a.a.) and having a molecular mass of 20.8kDa (Molecular size on SDS-PAGE will appear at approximately 18-40kDa). LIF is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
LIF Human, Yeast
LIF Human Recombinant, Yeast
LIF Mouse
Leukemia Inhibitory Factor Mouse Recombinant
The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques.
LIF Rat
Leukemia Inhibitory Factor Rat Recombinant
LIFR Human
Leukemia Inhibitory Factor Receptor Alpha Human Recombinant
LIFR Mouse
Leukemia Inhibitory Factor Receptor Alpha Mouse Recombinant
LIFR Mouse Recombinant produced in Baculovirus is a single glycosylated polypeptide chain containing 794 amino acids (44-828 aa) and having a molecular mass of 89.6kDa.
LIFR is fused to a 9 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
Introduction
Leukemia Inhibitory Factor (LIF) is a cytokine belonging to the interleukin-6 (IL-6) family. It is known for its ability to inhibit the differentiation of myeloid leukemic cells, hence its name . LIF is classified as a polyfunctional glycoprotein cytokine that can affect various cell types and tissues .
LIF exhibits several key biological properties:
- Expression Patterns: LIF is expressed in various tissues, including the trophectoderm of developing embryos .
- Tissue Distribution: It is found in multiple tissues such as bone, neural tissues, and the reproductive system .
- Key Properties: LIF influences cell growth, differentiation, and survival. It plays roles in bone metabolism, neural development, embryogenesis, and inflammation .
LIF has diverse biological functions:
- Primary Functions: It induces the terminal differentiation of myeloid leukemic cells, preventing their proliferation . It also promotes the growth and differentiation of various target cells .
- Role in Immune Responses: LIF is involved in immune responses by modulating the activity of macrophages and other immune cells .
- Pathogen Recognition: It plays a role in recognizing and responding to pathogens by influencing immune cell behavior .
LIF operates through several mechanisms:
- Binding Partners: LIF binds to the LIF receptor (LIFR), which forms a heterodimer with the gp130 receptor .
- Downstream Signaling Cascades: This binding activates multiple signaling pathways, including JAK/STAT, MAPK, and PI3K pathways . These cascades lead to changes in gene expression and cellular responses .
The expression and activity of LIF are tightly regulated:
- Transcriptional Regulation: LIF expression is controlled at the transcriptional level by various factors .
- Post-Translational Modifications: LIF undergoes glycosylation, which affects its stability and activity . Additionally, its signaling is modulated by proteins such as SOCS (suppressors of cytokine signaling) and PIAS (protein inhibitors of activated STAT) .
LIF has several applications in biomedical research and therapy:
- Biomedical Research: It is used in stem cell research to maintain the pluripotency of embryonic stem cells .
- Diagnostic Tools: LIF can serve as a biomarker for certain cancers, such as pancreatic cancer .
- Therapeutic Strategies: LIF has potential therapeutic applications in treating infertility, cancer, and tissue regeneration .
LIF plays crucial roles throughout the life cycle:
