LIF Rat
Leukemia Inhibitory Factor Rat Recombinant
Cat. No.
BT19534
Source
Escherichia Coli.
Synonyms
Leukemia inhibitory factor, Cholinergic neuronal differentiation factor, Lif.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Leukemia Inhibitory Factor (LIF) Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.8 kDa. The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Leukemia Inhibitory Factor (LIF) is a protein that plays a crucial role in maintaining embryonic stem cells by preventing them from differentiating. It has various functions, including aiding in the development of nerve cells, influencing stem cell versatility, regulating bone and fat metabolism, stimulating the growth of specific cell lines, and promoting the production of megakaryocytes (cells that produce platelets). Interestingly, the LIF protein in humans and mice shares a significant similarity in their structure, with 78% of their amino acid sequences being identical.
Description
This product is a laboratory-produced version of Rat Leukemia Inhibitory Factor (LIF). It is a single chain protein that is not glycosylated, meaning it lacks sugar molecules that are typically attached to proteins. It consists of 180 amino acids and has a molecular weight of 19.8 kDa. The purification process involves specialized chromatography techniques to ensure its high quality.
Physical Appearance
It appears as a white powder that has been freeze-dried and sterilized through filtration.
Formulation
The Rat LIF product is freeze-dried from a concentrated solution in a phosphate buffer (PBS) with a pH of 7.4. The solution undergoes filtration through a 0.2 micrometer filter before the freeze-drying process.
Solubility
To reconstitute the freeze-dried LIF, it is recommended to dissolve it in sterile water at a concentration of at least 100 micrograms per milliliter (µg/ml). Once reconstituted, it can be further diluted in other aqueous solutions as needed.
Stability
While the freeze-dried LIF remains stable at room temperature for up to 3 weeks, it is best stored in a dry environment below -18°C. After reconstitution, the LIF solution can be stored at 4°C for 2-7 days. For long-term storage, freezing below -18°C is recommended, ideally with the addition of a carrier protein such as HSA or BSA at a concentration of 0.1% to maintain its stability. Avoid repeated freezing and thawing of the product.
Purity
The purity of this product exceeds 96%, as confirmed by two analytical methods: High-Performance Liquid Chromatography (RP-HPLC) and SDS-PAGE.
Biological Activity
The biological activity of this rat LIF product is assessed by its ability to stimulate the differentiation of M1 myeloid leukemia cells. The minimum concentration of rat LIF required to observe a detectable effect in this assay is 0.5 nanograms per milliliter (ng/mL).
Synonyms
Leukemia inhibitory factor, Cholinergic neuronal differentiation factor, Lif.
Source
Escherichia Coli.
Amino Acid Sequence
SPLPITPVNA TCAIRHPCHG NLMNQIKSQL AQLNGSANAL FISYYTAQGE PFPNNVDKLC APNMTDFPPF HANGTEKTKL VELYRMVTYL GASLTNITWD QKNLNPTAVS LQIKLNATTD VMRGLLSSVL CRLCNKYHVG HVDVPCVPDN SSKEAFQRKK LGCQLLGTYK QVISVLAQAF .
Product Science Overview
Structure and Production
Recombinant rat LIF (rrLIF) is a single, non-glycosylated polypeptide chain containing 180 amino acids, with a molecular mass of approximately 19.8 kDa . It is produced in Escherichia coli (E. coli) using recombinant DNA technology, which involves the insertion of the LIF gene into the bacterial genome. The bacteria then express the LIF protein, which is subsequently purified using chromatographic techniques .
Biological Functions
LIF is known for its ability to promote the long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation . This property makes it invaluable in stem cell research, where maintaining the pluripotency of stem cells is essential. Additionally, LIF has several other biological activities, including:
- Cholinergic Neuron Differentiation: LIF promotes the differentiation of cholinergic neurons, which are essential for the proper functioning of the nervous system .
- Control of Stem Cell Pluripotency: LIF helps in maintaining the pluripotency of stem cells, allowing them to differentiate into various cell types .
- Bone and Fat Metabolism: LIF plays a role in regulating bone and fat metabolism, contributing to overall metabolic homeostasis .
- Mitogenesis: LIF stimulates the mitogenesis of certain factor-dependent cell lines, promoting cell division and growth .
- Megakaryocyte Production: LIF enhances the production of megakaryocytes, which are precursors to platelets, essential for blood clotting .
Applications in Research
Recombinant rat LIF is widely used in cell culture applications, particularly in the culture of embryonic stem cells. It is biologically active and suitable for maintaining the undifferentiated state of stem cells . Researchers utilize rrLIF to study various aspects of stem cell biology, including differentiation, pluripotency, and cellular signaling pathways.
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