LIF Human, His
Escherichia Coli.
CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA.
Greater than 95.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
LIF Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 23-202) containing 189 amino acids including a 9 a.a N-terminal His tag. The total molecular mass is 20.9kDa (calculated).
Product Specs
CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA.
Escherichia Coli.
MKHHHHHHAS PLPITPVNAT CAIRHPCHNN LMNQIRSQLA QLNGSANALF ILYYTAQGEP FPNNLDKLCG PNVTDFPPFH ANGTEKAKLV ELYRIVVYLG TSLGNITRDQ KILNPSALSL HSKLNATADI LRGLLSNVLC RLCSKYHVGH VDVTYGPDTS GKDVFQKKKL GCQLLGKYKQ IIAVLAQAF.
Product Science Overview
The human recombinant LIF with a His tag is produced in Escherichia coli (E. coli) expression systems. This recombinant protein is a single, non-glycosylated polypeptide chain consisting of 189 amino acids, including a 9-amino acid N-terminal His tag . The molecular weight of this recombinant protein is approximately 20.9 kDa .
LIF has several important functions:
- Stem Cell Maintenance: LIF is essential for the long-term maintenance of embryonic stem cells by preventing their spontaneous differentiation .
- Neuronal Differentiation: It promotes the differentiation of cholinergic neurons .
- Bone and Fat Metabolism: LIF plays a role in regulating bone and fat metabolism .
- Immune Response: It can be up-regulated by pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNFα) and interleukin-17 (IL-17), and elevated levels of LIF have been observed in conditions like rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis .
Recombinant human LIF is widely used in research and biotechnology for various applications:
- Stem Cell Research: It is used to maintain the pluripotency of embryonic stem cells in culture .
- Functional Assays: LIF is employed in functional assays to study its effects on cell proliferation and differentiation .
- Immunohistochemistry and ELISA: It is used in immunohistochemistry and enzyme-linked immunosorbent assays (ELISA) to detect and quantify LIF levels in biological samples .
The lyophilized recombinant human LIF should be reconstituted in sterile, distilled water or an appropriate buffered solution containing 0.1% bovine serum albumin (BSA) to regain full activity . The reconstituted protein should be apportioned into working aliquots and stored at -20°C to avoid repeated freeze-thaw cycles . The lyophilized protein can be stored at 2-8°C, preferably desiccated .
