Viral Antigens
Product List
ACE Human
Angiotensin Converting Enzyme Human Recombinant
ACE Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 1235 amino acids (30-1256 a.a.) and having a molecular mass of 142kDa.
ACE is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
CoV HKU1 Human
Coronavirus HKU1 Nucleoprotein Human Recombinant
Recombinant Human Coronavirus HKU1 Nucleoprotein is full length protein except the predicted signal peptide of the first 30 aa produced in E.coli and migrate at 50kDa.
The CoV HKU1 is fused to a 6xHis tag at its C terminal and purified by proprietary chromatographic technique.
Escherichia Coli.
CoV OC43 Human
Coronavirus OC43 Nucleoprotein Human Recombinant
Recombinant Human Coronavirus OC43 Nucleoprotein is full length protein except the predicted signal peptide of the first 30 aa produced in E. coli and migrate at 50kDa.
The CoV OC43 is fused to a 6xHis tag at its C terminal and purified by proprietary chromatographic technique.
Escherichia Coli.
CoV-2 3CL
Coronavirus 2019 3CL Protease Recombinant
Recombinant Coronavirus 2019 3CL Protease having a Mw of approximately 33.8 kDa was purified from E. coli. The CoV-2 3CL contains 306 amino acids and purified by proprietary chromatographic technique.
Escherichia Coli.
Filtered White lyophilized (freeze-dried) powder.
CoV-2 Envelope (1-75)
Coronavirus 2019 Envelope (1-75 a.a.), Recombinant
The E.coli derived recombinant protein contains the Coronavirus 2019 CoV-2 Envelope, Wuhan-Hu-1 strain, amino acids 1-75 fused to GST-tag at N-terminal & His-tag at C-terminal having a calculated Mw of 36.8 kDa and migrating between 33-35 umder reducing condition.
E.Coli
CoV-2 Membrane Env.
Coronavirus 2019 Membrane Envelope, Recombinant
The E.Coli derived recombinant fusion protein contains the Coronavirus 2019 Full-Length Membrane and Envelope proteins, Wuhan-Hu-1 strain, having a Mw of 34.2 kDa fused to His tag at C-terminal.
CoV-2 N (127 a.a.)
Coronavirus 2019 Nucleocapsid (127 a.a.), Recombinant
The E.Coli derived recombinant protein contains the Coronavirus 2019 C-terminal region 127 a.a. from the Nucleocapsid protein and fused to GST-6xHis tag at N-terminal and having a Mw. of 39.4 kDa.
CoV-2 N (1-419)
Coronavirus 2019 Nucleocapsid (1-419 a.a.), Recombinant
The E.Colie derived recombinant protein contains the Coronavirus 2019 CoV-2 Nucleocapsid Phosphoprotein, Wuhan-Hu-1 strain, amino acids (1-419) having a Mw of 46.4 kDa and fused to 6xHis tag at C-terminal.
E.Coli
CoV-2 N (1-419), Biotin
Coronavirus 2019 Nucleocapsid (1-419 a.a.), Biotinylated Recombinant
The HEK293 derived recombinant biotinylated protein contains the Coronavirus 2019 CoV-2 Nucleocapsid, Wuhan-Hu-1 strain, amino acids 1-419 fused to His-Avi tag at C-terminal.
HEK293 Cells.
CoV-2 N (1-419), HEK
Coronavirus 2019 Nucleocapsid (1-419 a.a.), Recombinant, HEK
The HEK293 derived recombinant protein contains the Coronavirus 2019 CoV-2 Nucleocapsid, Wuhan-Hu-1 strain, amino acids 1-419 fused to His tag at C-terminal having a calculated Mw of 47.3 kDa and migrating between 60-65 due to glycosylation.
HEK293 Cells.
Introduction
Severe Acute Respiratory Syndrome (SARS) is a viral respiratory illness caused by a coronavirus known as SARS-CoV. It was first identified in 2003 during an outbreak that began in China and spread to other countries . SARS-CoV belongs to the family Coronaviridae, which is divided into four genera: Alpha, Beta, Gamma, and Delta coronaviruses . SARS-CoV is classified under the Betacoronavirus genus.
Key Biological Properties: SARS-CoV is an enveloped, positive-sense, single-stranded RNA virus. It has a crown-like appearance due to spike proteins on its surface .
Expression Patterns and Tissue Distribution: SARS-CoV primarily infects the respiratory tract, but it can also affect other organs such as the gastrointestinal tract, liver, and kidneys . The virus binds to the angiotensin-converting enzyme 2 (ACE2) receptor, which is widely distributed in various tissues, including the lungs, heart, and intestines .
Primary Biological Functions: The primary function of SARS-CoV is to replicate within host cells. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions .
Role in Immune Responses and Pathogen Recognition: SARS-CoV triggers an immune response by activating various immune cells and signaling pathways. The spike protein of the virus is recognized by the host’s immune system, leading to the production of neutralizing antibodies .
Mechanisms with Other Molecules and Cells: SARS-CoV enters host cells by binding to the ACE2 receptor and undergoing proteolytic cleavage by host cell proteases such as TMPRSS2 . This facilitates viral entry and fusion with the host cell membrane.
Binding Partners and Downstream Signaling Cascades: The binding of SARS-CoV to ACE2 triggers downstream signaling cascades that modulate immune responses and inflammation. The virus can also evade immune detection by interfering with interferon signaling pathways .
Transcriptional Regulation: The expression of SARS-CoV genes is tightly regulated by viral and host factors. Transcription factors such as SP1 and HNF4α play crucial roles in regulating the expression of the ACE2 receptor, which is essential for viral entry .
Post-Translational Modifications: SARS-CoV proteins undergo various post-translational modifications, including phosphorylation, glycosylation, and ubiquitination, which are critical for viral replication and immune evasion .
Biomedical Research: SARS-CoV has been extensively studied to understand viral pathogenesis and host immune responses. This research has led to the development of diagnostic tools and therapeutic strategies .
Diagnostic Tools: Techniques such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) are used to detect SARS-CoV infection .
Therapeutic Strategies: Antiviral drugs, monoclonal antibodies, and vaccines have been developed to combat SARS-CoV infection. These therapeutic strategies target various stages of the viral life cycle .
Throughout the Life Cycle: SARS-CoV plays a critical role in the viral life cycle, from initial infection to replication and assembly of new virions. The virus hijacks the host’s cellular machinery to produce viral RNA and proteins, leading to the assembly of new virions . The N protein of SARS-CoV is essential for packaging the viral RNA into new virions and facilitating their release from host cells .
