Viral Antigens
Product List
HCV 4th Generation
Hepatitis C Virus 4th Generation Recombinant
The E.Coli derived HCV fourth generation antigen recombinant protein contains medium size core (1-126aa), NS3 (226aa), NS4 (3 epitopes) & NS5 (3 epitopes). HCV 4th Generation migrates at 46kDa.
HCV 4th Generation 65kDa
Recombinant Hepatitis C Virus 4th Generation 65 kDa
HCV 8th Generation
Hepatitis C Virus 8th Generation Recombinant
The E.Coli derived HCV Eighth generation antigen recombinant mosaic fusion protein contains multiple gene including env – core - NS3 - NS4 – NS5 and covers genotype I, II and III. The protein size is about 80kDa with GST tag at N-terminal
HCV Cocktail
Hepatitis C Virus Cocktail Recombinant
Recombinant HCV cocktail contains the recombinant hepatitis C virus core, NS3, NS4, NS5 proteins.
HCV Combined
Hepatitis C Virus Combined Recombinant
The protein contains the HCV nucleocapsid, NS3 genotype 1b, NS4 genotype 1b and 1a, and NS5 genotype 1b and 1a immunodominant regions.
HCV Core 1-120
Hepatitis C Virus Nucleocapsid (core) 1-120 a.a. Recombinant
Recombinant HCV Core 1-120 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
HCV Core 16.8kDa
Hepatitis C Virus Nucleocapsid (core) 16.8kDa Recombinant
Recombinant HCV Core produced in E.coli is a non-glycosylated polypeptide chain having a molecular mass of 16.8 kDa and fused to a His tag at N-terminus.
Sterile filtered colorless solution.
HCV Core 169aa
Hepatitis C Virus Nucleocapsid (core) 169aa Recombinant
Recombinant HCV core protein genotype 1b produced in E.Coli, comprised of the large core peptide containing 169 a.a. which forms a dimer, fused to a 6xHis tag at C-terminus, having a total Mw of 38kDa and pI of 11.02. Recombinant HCV core protein genotype 1b was purified by proprietary chromatographic techniques.
HCV Core 22kDa
Hepatitis C Virus Core 22kDa Recombinant
The protein is fused with b-galactosidase (114 kDa) at N-terminus.
HCV Core 22kDa, Biotin
Hepatitis C Virus Core 22kDa, Biotin Recombinant
The Biotin labeled protein is fused with b-galactosidase (114 kDa) at N-terminus.
Introduction
Hepatitis C is a liver disease caused by the hepatitis C virus (HCV), a small, enveloped, positive-sense single-stranded RNA virus belonging to the genus Hepacivirus within the family Flaviviridae . HCV can cause both acute and chronic hepatitis, ranging from a mild illness lasting a few weeks to a serious, lifelong illness including liver cirrhosis and cancer . There are seven major genotypes and numerous subtypes of HCV, which vary in their geographical distribution and response to treatment .
HCV particles are spherical and range from 40 to 80 nanometers in diameter . The virus has a lipid membrane envelope embedded with two glycoproteins, E1 and E2, which play crucial roles in viral attachment and entry into host cells . HCV primarily infects hepatocytes in the liver but can also be found in other tissues such as lymph nodes and peripheral blood mononuclear cells . The virus exhibits significant genetic diversity, which contributes to its ability to evade the host immune system .
HCV’s primary function is to replicate within host cells. It hijacks the host’s cellular machinery to produce viral proteins and RNA . The virus plays a role in immune evasion by interfering with the host’s immune responses, including the inhibition of interferon signaling pathways . HCV also induces chronic inflammation, which can lead to liver fibrosis, cirrhosis, and hepatocellular carcinoma .
HCV enters host cells through interactions with several receptors, including the low-density lipoprotein receptor (LDLr), heparan sulfate proteoglycans (HSPGs), scavenger receptor B1 (SRB1), and CD81 . Once inside the cell, the virus releases its RNA genome, which is translated into a single polyprotein. This polyprotein is then cleaved into structural and non-structural proteins that are essential for viral replication . The virus also manipulates host cell signaling pathways to create a favorable environment for its replication .
The expression and activity of HCV are regulated at multiple levels. Transcriptional regulation involves the interaction of viral RNA with host cell factors that enhance or inhibit viral replication . Post-translational modifications, such as phosphorylation and ubiquitination, also play critical roles in regulating the stability and function of viral proteins . Additionally, HCV can modulate host immune responses to promote viral persistence .
HCV research has led to significant advancements in understanding viral pathogenesis and developing therapeutic strategies. Direct-acting antivirals (DAAs) have revolutionized HCV treatment, offering high cure rates with fewer side effects compared to previous therapies . HCV is also used as a model to study RNA virus replication and host-virus interactions . Diagnostic tools, such as serological assays and nucleic acid tests, are essential for detecting HCV infection and monitoring treatment response .
HCV’s life cycle begins with the attachment of the virus to host cell receptors, followed by entry into the cell via endocytosis . Once inside, the viral RNA is released and translated into a polyprotein, which is processed into functional viral proteins . These proteins facilitate viral RNA replication and assembly of new virions, which are then released to infect other cells . Throughout its life cycle, HCV interacts with various host factors to ensure its replication and persistence .
