HCV Core 169aa
Greater than 85.0% as determined by SDS-PAGE.
Description
Recombinant HCV core protein genotype 1b produced in E.Coli, comprised of the large core peptide containing 169 a.a. which forms a dimer, fused to a 6xHis tag at C-terminus, having a total Mw of 38kDa and pI of 11.02. Recombinant HCV core protein genotype 1b was purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Hepatitis C Virus (HCV) is a significant global health concern, affecting millions of people worldwide. The virus is known for causing both acute and chronic hepatitis, which can lead to severe liver damage, cirrhosis, and hepatocellular carcinoma. One of the critical components of HCV is the nucleocapsid, also known as the core protein, which plays a vital role in the virus’s structure and replication.
The HCV core protein is a structural protein encoded by the HCV genome. It is the first protein translated from the viral RNA and is essential for the formation of the viral nucleocapsid. The core protein is approximately 169 amino acids long and is highly conserved among different HCV genotypes . It is responsible for packaging the viral RNA and forming the nucleocapsid, which is crucial for the assembly and release of new viral particles.
The core protein also interacts with various host cell components, influencing cellular processes such as apoptosis, lipid metabolism, and immune response modulation. These interactions are essential for the virus’s ability to establish and maintain chronic infection .
Recombinant HCV core protein is produced using recombinant DNA technology, where the gene encoding the core protein is inserted into an expression vector and introduced into a host cell, such as Escherichia coli. The host cells then produce the core protein, which can be purified and used for various research and diagnostic purposes .
Recombinant HCV core protein is commonly used in enzyme-linked immunosorbent assays (ELISA) and Western blotting to detect HCV antibodies in patient samples. It is also used in vaccine development and studies aimed at understanding the virus’s structure and function .
- Diagnostic Tools: Recombinant HCV core protein is a crucial component in diagnostic assays for detecting HCV infection. These assays help identify infected individuals and monitor the effectiveness of antiviral treatments.
- Vaccine Development: The core protein is a target for vaccine development due to its essential role in the virus’s life cycle. Researchers are exploring various strategies to elicit a robust immune response against the core protein, which could provide protection against HCV infection .
- Therapeutic Research: Understanding the interactions between the core protein and host cell components can lead to the development of novel antiviral therapies. By targeting these interactions, researchers aim to disrupt the virus’s ability to replicate and establish chronic infection .
