HCV Core Genotype-1a

Hepatitis C Virus Core Genotype-1a Recombinant
Cat. No.
BT11677
Source
Synonyms
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Purity
HCV Core Genotype-1a protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.coli derived recombinant protein fused to a His tag at C-terminus contains the HCV core nucleocapsid immunodominant regions, amino acids 2-119.

Product Specs

Introduction
Hepatitis C virus (HCV) is a small (50nm), enveloped virus with a single-stranded, positive-sense RNA genome. It belongs to the family Flaviviridae. HCV replicates rapidly, producing approximately one trillion particles daily in an infected individual. The virus's RNA polymerase lacks proofreading ability, resulting in a high mutation rate. This characteristic allows HCV to evade the host's immune system effectively. HCV is classified into six genotypes (1-6), with multiple subtypes within each genotype. The prevalence and distribution of these genotypes vary globally. Genotype determination is crucial for clinical management as it influences the response to interferon-based therapy and the required treatment duration. Genotypes 1 and 4 show a lower response rate to interferon-based treatment compared to genotypes 2, 3, 5, and 6.
Description
This recombinant protein is derived from E. coli and contains amino acids 2-119 of the HCV core nucleocapsid immunodominant region. It is fused to a His tag at its C-terminus.
Purity
The purity of HCV Core Genotype-1a protein is greater than 95% as determined by 10% SDS-PAGE and Coomassie blue staining.
Formulation
The protein is supplied in a buffer containing 1.5M urea, 25mM Tris-HCl (pH 8.0), 0.2% Tween-20, and 50% glycerol.
Stability
HCV Core Genotype-1a protein remains stable at 4°C for one week. However, for long-term storage, it is recommended to store the protein below -18°C. Avoid repeated freeze-thaw cycles.
Purification Method
HCV Core Genotype-1a protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of HCV-infected individuals.

Product Science Overview

Introduction

Hepatitis C virus (HCV) is a significant global health concern, affecting millions of people worldwide. It is a bloodborne virus that primarily infects the liver, leading to chronic liver diseases such as cirrhosis and hepatocellular carcinoma. HCV is classified into seven major genotypes, with genotype 1 being the most prevalent globally. Within genotype 1, there are subtypes 1a and 1b, which are of particular interest due to their varying responses to antiviral treatments.

Structure and Function of HCV Core Protein

The HCV core protein is a structural component of the virus, playing a crucial role in the formation of the viral nucleocapsid. It is encoded by the 5’ end of the HCV genome and is highly conserved among different HCV genotypes. The core protein is involved in various functions, including viral RNA packaging, interaction with host cell proteins, and modulation of host immune responses .

Genotype-1a and Its Significance

Genotype-1a is one of the most common subtypes of HCV, particularly in North America and Europe. It is known for its relatively high resistance to certain antiviral therapies compared to other genotypes. This resistance has driven extensive research into understanding the genetic and structural characteristics of genotype-1a, leading to the development of more effective treatment regimens .

Recombinant HCV Core Genotype-1a

Recombinant HCV core genotype-1a refers to the artificially engineered version of the core protein, which is used in various research and clinical applications. Recombinant proteins are produced through genetic engineering techniques, where the gene encoding the HCV core protein is inserted into an expression system, such as bacteria or mammalian cells, to produce large quantities of the protein.

Applications of Recombinant HCV Core Genotype-1a
  1. Vaccine Development: Recombinant HCV core proteins are used in the development of vaccines aimed at eliciting an immune response against HCV. These vaccines are designed to stimulate the production of antibodies and T-cell responses that can target and neutralize the virus .
  2. Diagnostic Tools: Recombinant core proteins are utilized in diagnostic assays to detect HCV infections. These assays can identify antibodies against the core protein in the blood of infected individuals, aiding in the diagnosis and monitoring of HCV infections .
  3. Research: Recombinant HCV core genotype-1a is widely used in research to study the virus’s biology, including its replication, interaction with host cells, and immune evasion mechanisms. This research is crucial for developing new antiviral therapies and understanding the virus’s pathogenesis .

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