CoV-2 Membrane Env.
E.Coli
Protein is >90% pure as determined SDS-PAGE.
Description
The E.Coli derived recombinant fusion protein contains the Coronavirus 2019 Full-Length Membrane and Envelope proteins, Wuhan-Hu-1 strain, having a Mw of 34.2 kDa fused to His tag at C-terminal.
Product Specs
In December 2019, a novel coronavirus, designated 2019-nCoV, emerged in Wuhan, China, causing viral pneumonia in humans. This virus was linked to a seafood market in the city.
Genetic analysis revealed that 2019-nCoV shares 87% sequence identity with the bat-derived SARS-CoV-2 identified in Zhoushan, eastern China, in 2018. Notably, both viruses possess similar receptor-binding domains (RBDs) despite some amino acid variations. This suggests that 2019-nCoV might utilize the human ACE2 receptor (angiotensin-converting enzyme 2) for cell entry, similar to SARS-CoV.
While bats are considered the likely reservoir of 2019-nCoV, researchers hypothesize that an intermediate animal host, potentially from the seafood market, played a role in its transmission to humans. Recombination analysis indicates that the virus's spike glycoprotein might be a product of recombination between a bat coronavirus and an unidentified coronavirus.
This recombinant fusion protein is produced in E. coli and consists of the full-length Membrane (M) and Envelope (E) proteins of the Wuhan-Hu-1 strain of Coronavirus 2019. The protein has a molecular weight of 34.2 kDa and includes a C-terminal His tag for purification purposes.
This CoV-2 Membrane Envelope protein solution is supplied in a buffer solution of 1x PBS (phosphate-buffered saline).
The CoV-2 Membrane Envelope fusion protein is shipped to you frozen on ice packs to maintain its stability. Upon receiving the protein, it's crucial to store it at -20°C for long-term preservation.
The purity of this protein is greater than 90%, as determined by SDS-PAGE analysis.
E.Coli
Product Science Overview
The Coronavirus 2019 Membrane Envelope, Recombinant, refers to the recombinant form of the membrane (M) and envelope (E) proteins of the SARS-CoV-2 virus, which is responsible for the COVID-19 pandemic. These proteins play crucial roles in the virus’s structure, assembly, and pathogenesis.
The membrane (M) protein is the most abundant structural protein in the coronavirus. It spans the viral envelope and is responsible for maintaining the shape of the virus. The M protein interacts with other structural proteins, such as the spike (S) and nucleocapsid (N) proteins, to facilitate the assembly and budding of new virions .
The envelope (E) protein is a small, integral membrane protein involved in several aspects of the virus’s life cycle, including assembly, budding, envelope formation, and pathogenesis . It functions as an ion channel and interacts with both viral and host cell proteins to modulate the virus’s replication and release .
Recombinant forms of the M and E proteins are produced using genetic engineering techniques. These recombinant proteins are used in various research applications, including the development of vaccines and diagnostic assays. By expressing these proteins in a controlled environment, researchers can study their structure, function, and interactions in detail .
The M and E proteins are highly conserved among coronaviruses, making them potential targets for vaccine development. DNA vaccines expressing the M and E proteins have shown promise in preclinical studies. These vaccines can elicit cellular immune responses, which are crucial for long-term immunity against the virus . Although these vaccines may not induce robust humoral immunity, they can provide partial protection and are valuable in optimizing vaccination strategies .
