CoV HKU1 Human
Escherichia Coli.
Protein is >90% pure as determined by 10% PAGE (coomassie staining).
Description
Recombinant Human Coronavirus HKU1 Nucleoprotein is full length protein except the predicted signal peptide of the first 30 aa produced in E.coli and migrate at 50kDa.
The CoV HKU1 is fused to a 6xHis tag at its C terminal and purified by proprietary chromatographic technique.
Product Specs
Human coronavirus HKU1 (CoV-HKU1) is one of seven coronaviruses known to cause respiratory illnesses in humans. The other six are CoV-229E, CoV-NL63, MERS-CoV, SARS-CoV (the virus responsible for the 2002-2003 SARS outbreak), SARS-CoV-2 (the virus responsible for the COVID-19 pandemic), and OC43. CoV-229E, NL63, CoV-HKU1, and OC43 typically cause mild, cold-like symptoms. These four viruses, along with MERS-CoV, SARS-CoV, and SARS-CoV-2, belong to a larger family of viruses called coronaviruses. Coronaviruses are enveloped viruses, meaning they are surrounded by a lipid membrane. They are also positive-sense, single-stranded RNA viruses, meaning their genetic material is in the form of RNA rather than DNA. CoV-HKU1, like other beta-coronaviruses, has a shorter spike-like protein called hemagglutinin esterase. CoV-HKU1 infects human cells by binding to the N-acetyl9-O-acetyl neuraminic acid receptor.
Recombinant Human Coronavirus HKU1 Nucleoprotein is a full-length protein expressed in E. coli bacteria. The protein lacks the first 30 amino acids that make up the predicted signal peptide and has a molecular weight of 50 kDa. It includes a 6xHis tag attached to the C-terminus to facilitate purification using a proprietary chromatographic technique.
CoV HKU1 protein is supplied in a solution containing phosphate-buffered saline (PBS) and 25mM potassium carbonate (K2CO3).
For short-term storage (up to 4 weeks), the protein solution should be stored at 4°C. For long-term storage, the solution should be stored at -20°C. It is recommended to add a carrier protein, such as albumin (HSA or BSA) at a concentration of 0.1%, to the solution before freezing. Repeated freezing and thawing of the protein solution should be avoided.
The purity of the CoV-HKU1 protein is greater than 90%, as determined by SDS-PAGE (polyacrylamide gel electrophoresis) followed by Coomassie blue staining.
This recombinant CoV-HKU1 protein is suitable for use in immunoassays, a type of biochemical assay that utilizes antibodies to detect and quantify target molecules.
Escherichia Coli.
SERNYQTFNR GRKTQPKFTV STQPQGNTIP HYSWFSGITQ FQKGRDFKFS DGQGVPIAFG VPPSEAKGYW YRHSRRSFKT ADGQQKQLLP RWYFYYLGTG PYANASYGES LEGVFWVANH QADTSTPSDV SSRDPTTQEA IPTRFPPGTI LPQGYYVEGS GRSASNSRPG SRSQSRGPNN RSLSRSNSNF RHSDSIVKPD ADEIANLVL AKLGKESKPQ QVTKQNAKEI RHKILTKPRQ KRTPNKHCNV QQCFGKRGPS QNFGNAEMLK LGTNDPQFPI LAELAPTPGA FFFGSKLDLV KRDSEADSPV KDVFELHYSG SIRFDSTLPG FETIMKVLEE NLNAYVNSNQ NTDSDSLSSK PQRKRGVKQL PEQFDSLNLS AGTQHISNDF TPEDHSLLAT LDDPYVEDSV A
Product Science Overview
Coronavirus HKU1 was discovered through retrospective investigations of nasopharyngeal aspirates collected between 1995 and 2002 from symptomatic individuals across several continents . It is classified under the betacoronavirus genus, which also includes other notable viruses such as SARS-CoV and MERS-CoV.
The nucleoprotein of Coronavirus HKU1 plays a crucial role in the virus’s ability to replicate and transcribe its RNA genome. This protein is responsible for packaging the viral RNA into a helical ribonucleoprotein complex, which is essential for the virus’s replication cycle. The nucleoprotein also interacts with other viral and host proteins to facilitate the assembly and release of new virions.
The human recombinant nucleoprotein of Coronavirus HKU1 is a laboratory-produced version of the viral protein. This recombinant protein is used in various research applications, including the study of viral replication, the development of diagnostic assays, and the creation of potential vaccines. By producing the nucleoprotein recombinantly, researchers can obtain large quantities of the protein for experimental purposes without the need to culture the virus itself.
Research on the recombinant nucleoprotein of Coronavirus HKU1 has provided valuable insights into the virus’s biology and pathogenesis. Studies have shown that the nucleoprotein is a key target for the host immune response, making it a potential candidate for vaccine development . Additionally, the recombinant nucleoprotein is used in serological assays to detect antibodies against Coronavirus HKU1 in human samples, aiding in epidemiological studies and the monitoring of viral spread.
