CoV-2 N (1-419), HEK
HEK293 Cells.
Protein is >95% pure as determined SDS-PAGE.
Description
The HEK293 derived recombinant protein contains the Coronavirus 2019 CoV-2 Nucleocapsid, Wuhan-Hu-1 strain, amino acids 1-419 fused to His tag at C-terminal having a calculated Mw of 47.3 kDa and migrating between 60-65 due to glycosylation.
Product Specs
The novel coronavirus responsible for causing viral pneumonia, officially named 2019-nCoV (later designated as SARS-CoV-2), was first identified in December 2019 in Wuhan, Hubei province, China. Its emergence was linked to a local seafood market.
Genetic analysis revealed that 2019-nCoV shared a high degree of similarity (87%) with SARS-CoV-2 strains detected in bats in Zhoushan, eastern China, in 2018. The virus utilizes a similar receptor-binding domain (RBD) structure as the 2018 SARS-CoV, despite some amino acid variations. This similarity allows 2019-nCoV to bind to the human ACE2 receptor (angiotensin-converting enzyme 2).
While bats are considered the likely natural reservoir of 2019-nCoV, an intermediate animal host, potentially present at the seafood market, is suspected to have played a role in its transmission to humans. Research suggests that 2019-nCoV may have arisen from a recombination event within its spike glycoprotein, involving a bat coronavirus and another unknown coronavirus.
This recombinant protein is derived from HEK293 cells and encompasses amino acids 1 to 419 of the SARS-CoV-2 Nucleocapsid protein (Wuhan-Hu-1 strain). A C-terminal His tag is present for purification purposes. The calculated molecular weight is 47.3 kDa. However, due to glycosylation, the protein exhibits a migration pattern between 60-65 kDa on SDS-PAGE.
The CoV-2 Nucleocapsid protein is provided in a solution of 1x PBS (pH 7.4) containing 10% trehalose.
Lyophilized CoV-2 Nucleocapsid protein remains stable at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store the desiccated protein below -18°C. After reconstitution, the protein can be stored at 4°C for 2-7 days. For long-term storage, aliquot and store below -18°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
To prepare a working stock solution, add deionized water to the lyophilized pellet to achieve a concentration of approximately 0.5 mg/ml. Allow the pellet to dissolve completely.
The purity of the protein is determined to be greater than 95% as assessed by SDS-PAGE analysis.
HEK293 Cells.
Purified by Metal-Afinity chromatographic technique.
Product Science Overview
The Coronavirus 2019 Nucleocapsid (N) protein, specifically the segment spanning amino acids 1-419, is a critical component of the SARS-CoV-2 virus, which is responsible for the COVID-19 pandemic. This protein is often produced recombinantly in human embryonic kidney (HEK) cells for research and diagnostic purposes. The N protein plays a pivotal role in the viral life cycle, including the packaging of viral RNA and the assembly of new virions .
The N protein is an abundant RNA-binding protein that is essential for the encapsidation of the viral genome. It is composed of 419 amino acids and has a molecular weight of approximately 45.6 kDa . The protein is highly immunogenic, making it a valuable target for diagnostic assays and vaccine development . Structurally, the N protein contains multiple domains that facilitate its interaction with RNA and other viral components .
The N protein is involved in several critical stages of the SARS-CoV-2 life cycle. It assists in the packaging of the viral RNA genome into ribonucleoprotein complexes, which are then assembled into new virions . Additionally, the N protein plays a role in the regulation of viral RNA synthesis and the modulation of host cell responses . Its high abundance in virions and strong immunogenicity make it a key target for both diagnostic and therapeutic strategies .
Recombinant production of the N protein in HEK cells is a common method used to obtain large quantities of the protein for research and diagnostic purposes. HEK cells are preferred due to their ability to perform post-translational modifications that are similar to those in human cells, ensuring that the recombinant protein closely resembles its native form . The recombinant N protein is often tagged with a His-tag to facilitate purification and detection .
The recombinant N protein is widely used in various research and diagnostic applications. Its strong immunogenicity makes it an ideal candidate for the development of serological assays to detect antibodies against SARS-CoV-2 in patient samples . Additionally, the N protein is used in studies aimed at understanding the molecular mechanisms of SARS-CoV-2 infection and the host immune response . It also serves as a potential target for the development of antiviral drugs and vaccines .
