Chemokines
Product List
IL 8 Canine
Interleukin-8 Canine Recombinant
The IL8 is purified by proprietary chromatographic techniques.
IL 8 Human (1-72)
Interleukin-8 (1-72 a.a.) Human Recombinant (CXCL8)
The IL-8 is purified by proprietary chromatographic techniques.
IL 8 Human (1-77)
Interleukin-8 (1-77 a.a) Human Recombinant (CXCL8)
The IL-8 is purified by proprietary chromatographic techniques.
IL 8 Human (1-77), His
Interleukin-8 (1-77 a.a) Human Recombinant (CXCL8), His Tag
The IL-8 His is purified by proprietary chromatographic techniques.
IL 8 Human, Pichia
Interleukin-8 (1-77 a.a.) Human Recombinant, (CXCL8) Pichia
The IL-8 is purified by proprietary chromatographic techniques.
IL 8 Porcine
Interleukin-8 (1-72 a.a) Porcine Recombinant (CXCL8)
The IL8 is purified by proprietary chromatographic techniques.
IL 8 Rhesus Macaque
Interleukin-8 Rhesus Macaque Recombinant
The IL 8 Rhesus Macaque is purified by proprietary chromatographic techniques.
IL8 Canine, HEK
Interleukin-8, HEK Canine Recombinant
IL8 Canine, HEK Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 80 amino acids (28-101 a.a) and having a molecular mass of 9.4 kDa.
IL8 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
IL8 GST
Interleukin-8 (1-72) (CXCL8) Human Recombinant, GST Tag
Recombinant Human Interleukin-8 produced in E. coli containing 72 amino acids.
Recombinant Human Interleukin-8 is fused to GST tag at its N-terminus and purified by proprietary chromatographic technique.
Introduction
Interleukin 8 (CXCL8), also known as chemokine (C-X-C motif) ligand 8, is a member of the CXC chemokine family. It is a small cytokine produced by various cell types, including macrophages, epithelial cells, and endothelial cells . CXCL8 is primarily known for its role in chemotaxis, attracting neutrophils to sites of infection or inflammation .
Key Biological Properties: CXCL8 is a potent neutrophil chemoattractant and activator. It induces chemotaxis in target cells, primarily neutrophils, causing them to migrate toward the site of infection .
Expression Patterns: CXCL8 is expressed by a variety of cells, including monocytes, macrophages, neutrophils, T lymphocytes, epithelial cells, and fibroblasts . Its expression is upregulated in response to inflammatory stimuli.
Tissue Distribution: CXCL8 is found in various tissues, including periodontal fiber, granulocytes, beta cells, cartilage tissue, olfactory zone of nasal mucosa, monocytes, mucosa of paranasal sinus, bone marrow cells, sperm, and gallbladder .
Primary Biological Functions: CXCL8 plays a crucial role in the immune response by attracting neutrophils to sites of infection or injury . It also stimulates phagocytosis and promotes angiogenesis .
Role in Immune Responses: CXCL8 is involved in the recruitment and activation of neutrophils, which are essential for the innate immune response . It also plays a role in pathogen recognition and clearance.
Mechanisms with Other Molecules and Cells: CXCL8 interacts with its receptors, CXCR1 and CXCR2, on the surface of target cells . This interaction triggers a series of downstream signaling cascades that lead to various cellular responses, including chemotaxis, degranulation, and respiratory burst .
Binding Partners: CXCL8 binds to glycosaminoglycans and its receptors, CXCR1 and CXCR2 . These interactions are crucial for its biological activity.
Downstream Signaling Cascades: Upon binding to its receptors, CXCL8 activates several signaling pathways, including the G protein-coupled receptor signaling pathway, intracellular signal transduction, and chemokine-mediated signaling pathway .
Transcriptional Regulation: The expression of CXCL8 is regulated by various transcription factors, including NF-κB and AP-1 . These factors bind to the CXCL8 promoter and enhance its transcription in response to inflammatory stimuli.
Post-Translational Modifications: CXCL8 undergoes post-translational modifications, including cleavage to generate active isoforms . These modifications are essential for its biological activity.
Other Regulatory Mechanisms: The expression of CXCL8 is also regulated by miRNAs, such as miRNA-146a/b-5p, which indirectly repress its expression by silencing the expression of IRAK1 .
Biomedical Research: CXCL8 is widely studied in the context of inflammation, cancer, and autoimmune diseases . It serves as a biomarker for various inflammatory conditions and is a target for therapeutic interventions.
Diagnostic Tools: Elevated levels of CXCL8 are associated with various diseases, including cancer and inflammatory disorders . It is used as a diagnostic marker to assess disease severity and progression.
Therapeutic Strategies: Targeting the CXCL8-CXCR1/2 axis is a promising therapeutic approach for treating inflammatory diseases and cancer . Inhibitors of CXCL8 and its receptors are being developed and tested in clinical trials.
Development: CXCL8 plays a role in embryonic development by regulating the migration and activation of hematopoietic progenitor cells .
Aging: The expression of CXCL8 increases with age, contributing to age-related inflammation and immune dysregulation .
Disease: CXCL8 is implicated in various diseases, including cancer, autoimmune disorders, and chronic inflammatory conditions . It promotes tumor growth, metastasis, and angiogenesis in the tumor microenvironment .
