IL 8 Rhesus Macaque
Interleukin-8 Rhesus Macaque Recombinant
Cat. No.
BT15442
Source
Escherichia Coli.
Synonyms
IL-8, CXCL8, Monocyte-derived neutrophil chemotactic factor, MDNCF, T-cell chemotactic factor, Neutrophil-activating protein 1, NAP-1, Protein 3-10C, Granulocyte chemotactic protein 1, GCP-1, Monocyte-derived neutrophil-activating peptide, MONAP, Emoctakin, K60, NAF, LECT, LUCT, 3-10C, LYNAP, SCYB8, TSG-1, AMCF-I, b-ENAP
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
IL 8 Rhesus Macaque Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 79 amino acids and having a molecular mass of 9.1kDa.
The IL 8 Rhesus Macaque is purified by proprietary chromatographic techniques.
The IL 8 Rhesus Macaque is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Interleukin-8 (IL-8), also known as Neutrophil Chemotactic Factor, is a chemokine secreted by various cell types, including macrophages, epithelial cells, and endothelial cells, in response to an antigen. It plays a crucial role in the immune response by attracting neutrophils, a type of white blood cell, to the site of inflammation.
Description
IL-8 Rhesus Macaque Recombinant is a non-glycosylated protein produced in E. coli. It consists of a single polypeptide chain with 79 amino acids and has a molecular weight of 9.1kDa. This purified protein is obtained through proprietary chromatographic methods.
Physical Appearance
The product appears as a white, sterile, and lyophilized (freeze-dried) powder.
Formulation
The lyophilized IL-8 is prepared in a concentrated solution of PBS at pH 7.4, which undergoes filtration through a 0.2µm filter before freeze-drying.
Solubility
For reconstitution, it is advised to dissolve the lyophilized IL-8 in sterile 18M-cm H₂O to a minimum concentration of 100µg/ml. Further dilutions can be prepared using other aqueous solutions.
Stability
Lyophilized IL-8 remains stable at room temperature for up to 3 weeks; however, it is recommended to store it in a desiccated state below -18°C. Once reconstituted, IL-8 should be kept at 4°C for a maximum of 2-7 days. For long-term storage, freezing below -18°C is advised. Avoid repeated freeze-thaw cycles.
Purity
The purity of the product is determined to be greater than 97.0% using SDS-PAGE analysis.
Biological Activity
The biological activity of IL-8 Rhesus Macaque Recombinant is assessed through its ability to attract human peripheral blood neutrophils. This is measured within a concentration range of 50-150ng/ml, resulting in a Specific Activity ranging from 6,667 to 20,000IU/mg.
Synonyms
IL-8, CXCL8, Monocyte-derived neutrophil chemotactic factor, MDNCF, T-cell chemotactic factor, Neutrophil-activating protein 1, NAP-1, Protein 3-10C, Granulocyte chemotactic protein 1, GCP-1, Monocyte-derived neutrophil-activating peptide, MONAP, Emoctakin, K60, NAF, LECT, LUCT, 3-10C, LYNAP, SCYB8, TSG-1, AMCF-I, b-ENAP
Source
Escherichia Coli.
Amino Acid Sequence
AVLPRSAKEL RCECIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE PWVQRVVEKF VKRAENQNP
Product Science Overview
Genetic and Structural Information
The interleukin-8 gene is located on chromosome 5 in the Rhesus macaque (Macaca mulatta). This gene is highly conserved and shares approximately 94% identity with the human IL-8 gene . The recombinant form of Rhesus macaque IL-8 is structurally and functionally similar to its human counterpart, making it a valuable tool for research in primate models of inflammation.
Production and Purification
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