IL8 Canine, HEK

Interleukin-8, also known as IL-8 or C-X-C motif chemokine 8 (CXCL8), is a chemokine produced by various cell types, including macrophages, epithelial cells, and endothelial cells. It plays a crucial role in the immune response by acting as a chemical signal that attracts neutrophils to the site of inflammation. This chemotactic property has earned IL-8 the designation of Neutrophil Chemotactic Factor .

Interleukin-8, HEK Canine Recombinant is produced in Human Embryonic Kidney 293 (HEK293) cells. These cells are commonly used in biotechnology for the production of recombinant proteins due to their high transfection efficiency and ability to perform post-translational modifications. The recombinant IL-8 produced in HEK293 cells is a single, glycosylated polypeptide chain containing 80 amino acids, with a molecular mass of approximately 9.4 kDa .

The recombinant IL-8 is expressed with a six amino acid histidine tag at the C-terminus, which facilitates its purification through affinity chromatography. The amino acid sequence of the recombinant IL-8 includes the following residues: VSSELRCQCI KTHSTPFHPK YIKELRVIDS GPHCENSEII VKLFNGNEVC LDPKEKWVQK VVQIFLKKAE KQDPHHHHHH .

IL-8 is a potent chemotactic factor for neutrophils, playing a significant role in the inflammatory response. Upon encountering an antigen, macrophages phagocytose the particle and release chemokines, including IL-8, to signal other immune cells to the site of inflammation. This process is essential for the body’s defense against infections and injuries .

The recombinant IL-8 is provided as a sterile filtered, colorless solution. The formulation typically contains phosphate-buffered saline (pH 7.4) and 20% glycerol. For optimal stability, the solution should be stored at 4°C if used within 2-4 weeks. For longer storage periods, it is recommended to freeze the solution at -20°C and avoid multiple freeze-thaw cycles. Adding a carrier protein, such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA), can further enhance stability .

Interleukin-8, HEK Canine Recombinant is widely used in laboratory research to study the mechanisms of inflammation and immune response. It serves as a valuable tool for investigating the chemotactic properties of IL-8 and its role in various pathological conditions, including infections, autoimmune diseases, and cancer .

ProSpec’s IL-8 Canine Recombinant is intended for laboratory research use only. It is not suitable for use as a drug, agricultural or pesticidal product, food additive, or household chemical. Researchers handling this product should follow appropriate safety guidelines and protocols .