TFF3 Human, His

Trefoil Factor-3 (TFF3), also known as Intestinal Trefoil Factor (ITF), is a member of the trefoil factor family of peptides. These peptides are characterized by the presence of one or more trefoil domains, which are compact, stable structures containing three conserved disulfide bonds. TFF3 plays a crucial role in the maintenance and repair of the intestinal mucosa, promoting the mobility of epithelial cells during healing processes.

The human recombinant TFF3 protein with a His tag is a full-length protein expressed in HEK 293 cells. It consists of 94 amino acids and includes a His tag at the C-terminus for purification purposes . The His tag facilitates the purification of the protein using immobilized metal affinity chromatography (IMAC), ensuring high purity and yield.

TFF3 is primarily involved in the protection and repair of the gastrointestinal tract. It promotes epithelial restitution, a process where epithelial cells migrate to cover denuded areas of the mucosa, thus playing a critical role in mucosal healing. TFF3 also exhibits anti-apoptotic properties, helping to maintain the integrity of the epithelial barrier under stress conditions .

Recombinant TFF3 protein is widely used in research to study its role in gastrointestinal diseases, including inflammatory bowel disease (IBD) and colorectal cancer. It is also utilized in high-throughput screening assays to identify potential therapeutic agents that can modulate its activity .

The recombinant TFF3 protein is produced in HEK 293 cells, which are human embryonic kidney cells commonly used for protein expression due to their high transfection efficiency and ability to perform post-translational modifications. The protein is purified to a high degree of purity (>95%) and has an endotoxin level of less than 1 EU/µg, making it suitable for various biochemical assays .

The lyophilized recombinant TFF3 protein is stable at 2-8°C but should be stored at -20°C for long-term storage. Upon reconstitution, it is recommended to store the protein in working aliquots at -20°C to -80°C to avoid repeated freeze-thaw cycles, which can affect its stability and activity .