TFF3 Rat
Trefoil Factor-3 Rat Recombinant
Cat. No.
BT23427
Source
Escherichia Coli.
Synonyms
Trefoil factor 3, Intestinal trefoil factor, rITF, Polypeptide P1.B, rP1.B, Tff3, Itf.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
The Trefoil Factor-3 Rat was constructed as a recombinant protein with a 9 a.a C-terminal fusion of Flag-Tag (1 aa N-terminal+8 aa C-terminal). The TFF3 Rat produced in E.coli, is 7.7kDa protein containing a total of 68 amino acid residues.
Product Specs
Introduction
The TFF (Trefoil Factor) family of proteins is defined by the presence of at least one trefoil motif, a 40-amino acid domain containing three conserved disulfide bonds. These proteins are secreted by mucosal tissues in the gastrointestinal tract, where they provide protection against injury, stabilize the mucus layer, and promote epithelial healing. TFF2 specifically acts to inhibit gastric acid secretion and motility while also stabilizing mucus glycoproteins through interactions with carbohydrate side chains. TFF3, on the other hand, is involved in airway epithelial cell differentiation by inducing ciliogenesis, a process it facilitates largely through an epidermal growth factor receptor-dependent pathway. Importantly, TFF3 overexpression has been linked to the progression of hepatocellular carcinogenesis in both mice and humans, with its expression level correlating with tumor grade.
Description
This product is a recombinant Trefoil Factor-3 protein of rat origin (TFF3 Rat). It has been engineered with a C-terminal Flag-tag (9 amino acids total: 1 N-terminal + 8 C-terminal) and produced in E. coli. The resulting protein is 7.7 kDa in size and comprises 68 amino acid residues.
Physical Appearance
The product appears as a white lyophilized (freeze-dried) powder after filtration.
Formulation
The TFF3 protein undergoes filtration (0.4 µm) and is subsequently lyophilized at a concentration of 0.5 mg/ml in a buffer solution of 20 mM TRIS and 50 mM NaCl, pH 7.5.
Solubility
To prepare a working stock solution, it is recommended to add deionized water to the lyophilized pellet and allow it to dissolve completely until a concentration of approximately 0.5 mg/ml is achieved. Note: This product is not sterile. Before use in cell culture, it must be filtered through an appropriate sterile filter.
Stability
For long-term storage, keep the lyophilized protein at -20°C. After reconstitution, aliquot the product to minimize repeated freeze/thaw cycles. Reconstituted protein remains stable at 4°C for a limited period and has shown no significant changes over two weeks when stored at this temperature.
Purity
The purity of this product is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
Trefoil factor 3, Intestinal trefoil factor, rITF, Polypeptide P1.B, rP1.B, Tff3, Itf.
Source
Escherichia Coli.
Amino Acid Sequence
MQEFVGLSPS QCMVPANVRV DCGYPTVTSE QCNNRGCCFD SSIPNVPWCF KPLQETECT F DYKDDDDK.
Product Science Overview
Introduction
Trefoil Factor-3 (TFF3), also known as Intestinal Trefoil Factor (ITF), is a member of the trefoil factor family (TFF) peptides. These peptides are characterized by the presence of at least one trefoil motif, a 40-amino acid domain containing three conserved disulfide bonds . TFF3 is primarily expressed in the gastrointestinal mucosa and plays a crucial role in protecting and healing the epithelial lining.
Structure and Production
The recombinant form of TFF3 (Rat) is produced in Escherichia coli and is a 7.7 kDa protein consisting of 68 amino acid residues . The protein is constructed with a 9 amino acid C-terminal fusion of Flag-Tag, which aids in its identification and purification . The amino acid sequence of TFF3 (Rat) is as follows:
MQEFVGLSPS QCMVPANVRV DCGYPTVTSE QCNNRGCCFD SSIPNVPWCF KPLQETECT F DYKDDDDK
Biological Functions
TFF3 plays several vital roles in the body, including:
- Mucosal Protection: TFF3 stabilizes the mucus layer in the gastrointestinal tract, protecting the mucosa from various insults .
- Epithelial Healing: It promotes the healing of the epithelial lining by inducing ciliogenesis and promoting airway epithelial ciliated cell differentiation through an epidermal growth factor receptor-dependent pathway .
- Tumor Progression: Overexpression of TFF3 has been linked to the progression of hepatocellular carcinoma in both mice and humans .
Applications in Research
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