TFF3 Human
Trefoil Factor-3 Human Recombinant
Cat. No.
BT23285
Source
Escherichia Coli.
Synonyms
TFF-3, ITF, TFI, HITF, hP1.B, TFF3, Trefoil factor 3, Intestinal trefoil factor.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by RP-HPLC and SDS-PAGE analysis.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
TFF-3 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved interamolecular disulfide bonds and having a total molecular mass of 13.2kDa.
TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.
TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.
Product Specs
Introduction
The TFF family of proteins are known for possessing at least one copy of the trefoil motif, a 40-amino acid domain with 3 conserved disulfide bonds. These proteins, categorized as stable secretory proteins, are found in the gastrointestinal mucosa. Their roles involve safeguarding the mucosa against damage, maintaining the stability of the mucus layer, and influencing the healing process of the epithelium. Specifically, TFF2 plays a role in regulating gastric acid motility and secretion, while also stabilizing glycoproteins within the mucus gel by interacting with carbohydrate side chains. On the other hand, TFF3 promotes the formation of cilia and supports the differentiation of airway epithelial ciliated cells, potentially through a pathway involving the epidermal growth factor receptor. Notably, the overexpression of TFF3 has been linked to the progression of hepatocellular carcinogenesis in both humans and mice. This protein is commonly found in hepatocellular carcinoma, and its expression level is associated with the tumor grade.
Description
Recombinant human TFF-3, produced in E. coli, is a homodimeric polypeptide chain that is not glycosylated. It consists of two chains, each containing 59 amino acids, totaling 118 amino acids. A key feature of TFF-3 is the presence of a 40 amino acid trefoil motif, which includes three conserved intermolecular disulfide bonds. The total molecular mass of the protein is 13.2 kDa. The purification of recombinant human TFF-3 is carried out using specialized chromatographic methods.
Physical Appearance
The product is a sterile, white powder that has been lyophilized (freeze-dried) after filtration.
Formulation
The lyophilization process is performed on a concentrated solution (0.2µm filtered) of TFF-3 in phosphate-buffered saline (PBS) at a pH of 7.4.
Solubility
For reconstitution of the lyophilized TFF3, it is recommended to use sterile 18M-cm H2O. The minimum concentration should be 100µg/ml. This solution can be further diluted with other aqueous solutions as needed.
Stability
Lyophilized TFF3 demonstrates stability at room temperature for a period of 3 weeks; however, for long-term storage, it is recommended to store it in a dry environment below -18°C. Once reconstituted, TFF3 should be stored at 4°C for a maximum of 7 days. For extended storage, freezing below -18°C is advised. It is crucial to incorporate a carrier protein such as 0.1% HSA or BSA for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain protein integrity.
Purity
The purity of the protein is determined to be greater than 97.0% through analysis using RP-HPLC and SDS-PAGE techniques.
Biological Activity
A chemotaxis bioassay using human MCF-7 cells was used to determine the ED50, which was found to be less than 10µg/ml. This corresponds to a specific activity of greater than 100IU/mg.
Synonyms
TFF-3, ITF, TFI, HITF, hP1.B, TFF3, Trefoil factor 3, Intestinal trefoil factor.
Source
Escherichia Coli.
Amino Acid Sequence
EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF.
Product Science Overview
Structure and Expression
TFF3 exists in both monomeric (60 amino acids, 6.7 kDa) and dimeric (118 amino acids, 13.1 kDa) forms . The peptide is highly stable against proteolytic digestion, acid, and heat degradation due to its compact structure . TFF3 is primarily expressed by goblet cells in the gastrointestinal tract, but it is also found in the respiratory tract, biliary and breast ducts, uterus, and certain parts of the brain .
Functions
TFF3 plays a crucial role in the maintenance and repair of the gastrointestinal mucosa. It is involved in various physiological processes, including:
- Wound healing: TFF3 promotes epithelial regeneration and mucosal healing .
- Cell migration and proliferation: It aids in the migration and proliferation of epithelial cells, which is essential for tissue repair .
- Anti-apoptosis: TFF3 has been shown to inhibit apoptosis, contributing to cell survival .
- Mucin interaction: It interacts with mucins to stabilize the mucus layer, protecting the epithelial lining .
Clinical Significance
TFF3 has been studied for its potential diagnostic and therapeutic applications. Elevated levels of TFF3 have been observed in patients with gastrointestinal inflammation and ulceration . It is also being investigated for its role in various cancers, including colorectal, hepatocellular, and biliary tumors .
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
