TFF2 Human
Trefoil Factor-2 Human Recombinant
Cat. No.
BT23112
Source
Escherichia Coli.
Synonyms
TFF-2, Spasmolytic polypeptide, Spasmolysin, SML1, Trefoil factor 2, SP, TFF2.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
TFF-2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 106 amino acids (24-129) and having a total molecular mass of 12 kDa. TFF2 Human Recombinant includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds and was purified by proprietary chromatographic techniques.
Product Specs
Introduction
The TFF (Trefoil Factor) family proteins are known for possessing at least one trefoil motif, a 40-amino acid domain with 3 conserved disulfide bonds. These proteins are stable, secreted by the gastrointestinal mucosa, and play crucial roles in mucosal protection, mucus layer stabilization, and epithelial healing. TFF2, in particular, is recognized for its ability to regulate gastric acid secretion and motility while also stabilizing mucus glycoproteins through interactions with their carbohydrate side chains.
Description
Recombinant Human TFF2, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 106 amino acids (residues 24-129) with a molecular weight of 12 kDa. This protein contains a 40-amino acid trefoil motif characterized by three conserved intramolecular disulfide bonds and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
White lyophilized (freeze-dried) powder, sterile filtered.
Formulation
The TFF2 protein undergoes lyophilization from a 0.4 μm filtered solution at a concentration of 1 mg/mL in 1x PBS with a pH of 7.4.
Solubility
To reconstitute the lyophilized TFF2, it is recommended to dissolve it in sterile 18 MΩ-cm H2O to a concentration of at least 100 μg/mL. Further dilutions can be prepared in other aqueous solutions.
Stability
While lyophilized TFF2 remains stable at room temperature for up to 3 weeks, it is recommended to store it desiccated at temperatures below -18°C. Upon reconstitution, TFF2 should be stored at 4°C for 2-7 days or at temperatures below -18°C for future use. To ensure long-term storage stability, adding a carrier protein like HSA or BSA (0.1%) is advised. Avoid repeated freeze-thaw cycles.
Purity
The purity of TFF2 is determined using RP-HPLC and SDS-PAGE analysis and is found to be greater than 97%.
Biological Activity
The biological activity of TFF2 is assessed by its chemoattractant properties on human MCF-7 cells within a concentration range of 1.0-10.0 ng/mL.
Synonyms
TFF-2, Spasmolytic polypeptide, Spasmolysin, SML1, Trefoil factor 2, SP, TFF2.
Source
Escherichia Coli.
Amino Acid Sequence
EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKSVEDCHY.
Product Science Overview
Introduction
Trefoil Factor-2 (TFF2), also known as Spasmolysin or Spasmolytic Polypeptide, is a member of the trefoil factor family, which includes TFF1, TFF2, and TFF3. These proteins are characterized by the presence of at least one trefoil motif, a 40-amino acid domain containing three conserved disulfides .
Structure and Expression
Biological Functions
TFF2 has several important biological functions:
- Inhibition of Gastrointestinal Motility and Gastric Acid Secretion: TFF2 inhibits gastrointestinal motility and reduces gastric acid secretion, which helps protect the stomach lining from damage .
- Stabilization of Gastric Mucus: TFF2 may function as a structural component of gastric mucus by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains .
- Protection and Healing: TFF2 is believed to protect the mucosa from insults and aid in the healing of the epithelium .
Recombinant TFF2
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