TFF2 Human, His
Trefoil Factor-2 Human Recombinant, His Tag
Cat. No.
BT23199
Source
Escherichia Coli.
Synonyms
TFF-2, Spasmolytic polypeptide, Spasmolysin, SML1, Trefoil factor 2, SP, TFF2.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
TFF-2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 116 amino acids (24-129) which includes a 10 amino acid His Tag fused at N-terminus and having a total molecular mass of 13.2 kDa.
TFF2 Human Recombinant is purified by proprietary chromatographic techniques.
TFF2 Human Recombinant is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Proteins belonging to the TFF family are defined by the presence of at least one trefoil motif, a 40-amino acid domain containing 3 conserved disulfide bonds. These proteins are stable, secreted molecules found in the gastrointestinal mucosa, where they play protective roles against mucosal damage, contribute to the stability of the mucus layer, and influence epithelial healing. TFF2, in particular, acts to inhibit gastric acid secretion and motility, and it stabilizes mucus glycoproteins through interactions with carbohydrate side chains.
Description
Recombinant Human TFF-2, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 116 amino acids (residues 24-129), including a 10-amino acid His Tag fused at the N-terminus, resulting in a total molecular mass of 13.2 kDa. The purification of Recombinant Human TFF2 is achieved using proprietary chromatographic techniques.
Physical Appearance
White lyophilized (freeze-dried) powder.
Formulation
The TFF2 protein undergoes lyophilization from a 0.4 µm filtered solution. This solution has a concentration of 0.5 mg/ml and contains 20 mM Tris (pH 7.5) and 20 mM NaCl.
Solubility
To prepare a working stock solution, it is recommended to add deionized water to the lyophilized pellet, aiming for a concentration of approximately 0.5 mg/ml. Allow the pellet to dissolve completely. It is important to note that this product is not sterile. Before using it in cell culture, it is crucial to filter the product through an appropriate sterile filter.
Stability
Lyophilized TFF2 exhibits stability at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store it desiccated at temperatures below -18°C. After reconstitution, TFF2 should be stored at 4°C for a period of 2-7 days. For long-term storage after reconstitution, store below -18°C. To enhance stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). It is important to avoid repeated freeze-thaw cycles.
Purity
Purity greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
TFF-2, Spasmolytic polypeptide, Spasmolysin, SML1, Trefoil factor 2, SP, TFF2.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHAS EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKSVEDCHY.
Product Science Overview
Introduction
Structure and Expression
Function
TFF2 plays a crucial role in the gastrointestinal tract. It inhibits gastrointestinal motility and gastric acid secretion . Additionally, TFF2 is believed to function as a structural component of gastric mucus, stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains .
Applications
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