TFF1 Human, His

Trefoil Factor-1 Human Recombinant, His Tag
Cat. No.
BT23026
Source
Escherichia Coli.
Synonyms
TFF-1, TFF1, pS2, BCEI, HPS, HP1.A, pNR-2, D21S21, pS2 protein, Trefoil factor 1, Breast cancer estrogen-inducible protein.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TFF-1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids (25-84) which includes a 10 amino acid His Tag and having a total molecular mass of 7.9 kDa.
TFF-1 Human Recombinant is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Trefoil Factor peptides (TFF1, TFF2, and TFF3) are stable secretory proteins found in the gastrointestinal tract, particularly the gastric mucosa. They play crucial roles in protecting and repairing the intestinal mucosa. TFF1 is particularly important for the healthy development of the antral and pyloric gastric mucosa and acts as a tumor suppressor gene specific to the stomach. It helps create a protective mucous gel layer over the gastrointestinal mucosa, acting as a barrier against harmful substances. This protection from damage, stabilization of the mucus layer, and influence on epithelial healing are key functions of TFF1. Elevated levels of TFF1 are frequently observed in tumor cells. Furthermore, TFF1 interacts with the cell membrane of MCF-7 cells. In cases of inflammatory bowel disease, serum levels of both TFF1 and TFF2 are often elevated.
Description
Recombinant Human TFF-1, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 70 amino acids (residues 25-84), including a 10 amino acid His tag, resulting in a total molecular mass of 7.9 kDa. The purification of Recombinant Human TFF-1 is achieved using proprietary chromatographic techniques.
Physical Appearance
White, lyophilized (freeze-dried) powder after filtration.
Formulation
The TFF1 His Tag protein solution, with a concentration of 0.5 mg/ml, was filtered through a 0.4 μm filter. This solution, containing 20mM Tris pH-7.5 and 20mM NaCl, was then lyophilized to obtain the final product.
Solubility
To create a working stock solution, add deionized water to the lyophilized pellet, aiming for a concentration of approximately 0.5 mg/ml. Ensure complete dissolution of the pellet. This product is not sterile. Prior to use in cell culture, filter the solution through a sterile filter of appropriate pore size.
Stability
Lyophilized TFF1 His Tag remains stable at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store the lyophilized product desiccated at temperatures below -18°C. Once reconstituted, TFF1 His Tag can be stored at 4°C for 2-7 days. For long-term storage after reconstitution, store below -18°C. Adding a carrier protein, such as 0.1% HSA or BSA, is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 95.0% as determined by SDS-PAGE analysis.
Synonyms
TFF-1, TFF1, pS2, BCEI, HPS, HP1.A, pNR-2, D21S21, pS2 protein, Trefoil factor 1, Breast cancer estrogen-inducible protein.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHAS EAQTETCTVA PRERQNCGFP GVTPSQCANK GCCFDDTVRG VPWCFYPNTI DVPPEEECEF.

Product Science Overview

Introduction

Trefoil Factor-1 (TFF1), also known as pS2, is a small peptide that belongs to the trefoil factor family (TFF) of proteins. These proteins are characterized by the presence of one or more trefoil domains, which are compact, stable structures that play a crucial role in mucosal protection and repair. TFF1 is predominantly expressed in the epithelial cells of the stomach and is involved in maintaining the integrity of the gastrointestinal mucosa.

Biological Properties and Functions

TFF1 is a highly conserved protein that mediates mucosal repair by stimulating cell migration, inhibiting apoptosis, and promoting the barrier function of mucus . It forms a homodimer via a disulfide linkage, which is essential for its wound healing activity . TFF1 is also known to stabilize the mucous gel layer that covers the gastrointestinal mucosa, providing a physical barrier against various noxious agents .

Recombinant Expression

The recombinant form of TFF1 (Human Recombinant, His Tag) is produced using genetic engineering techniques. The gene encoding TFF1 is synthesized and expressed in host cells such as Escherichia coli or Brevibacillus choshinensis . The recombinant protein is then purified using affinity chromatography, typically involving a His tag, which allows for efficient purification.

Applications

Recombinant TFF1 has significant potential in therapeutic applications, particularly in the treatment of gastric damage and wound healing . Studies have shown that recombinant TFF1 produced by Brevibacillus choshinensis exhibits better wound healing capabilities compared to that produced by Escherichia coli . This enhanced activity is attributed to the glycosylation of the recombinant protein, which may contribute to its improved functionality .

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