OSM Rat
Oncostatin-M Rat Recombinant
Cat. No.
BT25436
Source
Escherichia Coli.
Synonyms
Oncostatin-M, OSM, OncoM.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
OSM Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 214 amino acids and having a molecular mass of 24.3kDa.
The OSM is purified by proprietary chromatographic techniques.
The OSM is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Oncostatin M, belonging to the cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6, is a growth regulator. This gene encodes for Oncostatin M which inhibits the proliferation of multiple tumor cell lines. It also regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells.
Description
Recombinant rat OSM, produced in E.coli, is a single, non-glycosylated polypeptide chain. It contains 214 amino acids, with a molecular weight of 24.3kDa. OSM is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The lyophilization of OSM protein was carried out from a 0.2 µm filtered concentrated solution in 1xPBS, at a pH of 7.4.
Solubility
For reconstituting the lyophilized Oncostatin M, it is recommended to use sterile 18MΩ-cm H2O at a concentration not less than 100µg/ml. This solution can be further diluted into other aqueous solutions.
Stability
Lyophilized Oncostatin M remains stable at room temperature for 3 weeks. However, it should be stored desiccated below -18°C. Upon reconstitution, Oncostatin M should be stored at 4°C for 2-7 days. For future use, store below -18°C. For long term storage, add a carrier protein (0.1% HSA or BSA). Avoid freeze-thaw cycles.
Purity
Purity is greater than 95.0% as determined by: (a) RP-HPLC analysis. (b) SDS-PAGE analysis.
Biological Activity
The ED50, determined by the dose-dependent stimulation of proliferation in rat C6 cells, is less than 5.0 µg/ml. This corresponds to a specific activity greater than 200 units/mg.
Synonyms
Oncostatin-M, OSM, OncoM.
Source
Escherichia Coli.
Amino Acid Sequence
KRGCSSSSPK LLSQLKSQAN ITGNTASLLE PYILHQNLNT LTLRAACTEH PVAFPSEDML RQLSKPDFLS TVHATLGRVW HQLGAFRQQF PKIQDFPELE RARQNIQGIR NNVYCMARLL HPPLEIPEPT QADSGTSRPT TTAPGIFQIK IDSCRFLWGY HRFMGSVGRV FEEWGDGSRR SR RHSPLWAW LKGDHRIRPS RSSQSAMLRS LVPR.
Product Science Overview
Structure and Production
Recombinant rat Oncostatin-M is typically produced in E. coli and consists of a single non-glycosylated polypeptide chain containing 214 amino acids . The molecular mass of this protein is approximately 24.3 kDa . The protein is fully biologically active, with an ED50 of less than 2.0 ng/ml, corresponding to a specific activity of more than 5.0 x 10^5 IU/mg .
Biological Functions
OSM plays a significant role in various physiological processes, including:
- Wound Healing: It is involved in the inflammatory response and tissue repair .
- Immune Response: OSM contributes to the body’s defense against viral infections .
- Hematopoiesis: It regulates the formation of blood cells in the bone marrow .
- Bone Development: OSM influences bone mass and osteoclast formation .
- Liver Homeostasis and Lipid Metabolism: It plays a role in maintaining liver function and regulating lipid metabolism .
Mechanism of Action
OSM exerts its effects through two heterodimeric receptors: GP130 with either the OSM receptor (OSMR) or the LIF receptor (LIFR) . The OSMR is expressed on osteoblasts, mesenchymal, and endothelial cells . Signaling through these receptors involves adaptor proteins like SHC1 and transducers like STAT3, which mediate the biological effects of OSM .
Clinical Implications
Dysregulated OSM production can lead to various pathologies, including:
- Bone Disorders: Excessive OSM can result in bone pathologies and defective muscle repair .
- Hematopoietic Stem Cell Mobilization: OSM acts as a brake to therapeutic hematopoietic stem cell mobilization, which can be problematic in conditions like diabetes .
- Cancer Progression: OSM and its receptor may play roles in the progression of certain cancers .
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