OSM Human, 195 a.a

The mature form of OSM is a single non-glycosylated polypeptide chain containing 195 amino acids . This recombinant protein is typically produced in E. coli and purified using high-performance liquid chromatography (HPLC) and other stringent purification methods to ensure high purity and bioactivity . The molecular weight of the mature OSM protein is approximately 22 kDa .

OSM is known for its role in regulating the growth and differentiation of various cell types. It has been shown to inhibit the proliferation of certain tumor cell lines while promoting the proliferation of normal cells . The biological activity of OSM is often assessed using cell proliferation assays, such as those involving human TF-1 cells, where it demonstrates significant activity at very low concentrations (ED50 < 2 ng/ml) .

Recombinant human OSM is used in various research applications, including:

• Cell culture studies: To investigate the effects of OSM on cell growth, differentiation, and signaling pathways.
• Bioactivity assays: To study the cytokine’s role in inflammation and immune responses.
• Cancer research: To explore its potential as a therapeutic agent due to its ability to inhibit tumor cell proliferation .

For optimal stability, the lyophilized recombinant OSM protein should be stored at -20°C to -70°C. After reconstitution, it can be stored at 2-8°C for short-term use or at -20°C to -70°C for long-term storage. It is important to avoid repeated freeze-thaw cycles to maintain its bioactivity .