OSM Human
Oncostatin-M Human Recombinant
Cat. No.
BT24976
Source
Escherichia Coli.
Synonyms
OSM, MGC20461.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Oncostatin-M Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 227 amino acids and having a molecular mass of 26kDa.
The OSM is purified by proprietary chromatographic techniques.
The OSM is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Oncostatin M, a member of the cytokine family that also includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6, is a growth regulator. This cytokine is known to inhibit the proliferation of various tumor cell lines and regulate the production of other cytokines like IL-6, G-CSF, and GM-CSF from endothelial cells.
Description
Recombinant Human Oncostatin-M, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 227 amino acids. With a molecular weight of 26kDa, it undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Lyophilized from a 1mg/ml solution in phosphate-buffered saline (PBS) at pH 7.4.
Solubility
To reconstitute the lyophilized Oncostatin M, it is recommended to dissolve it in sterile 18MΩ-cm H2O to a concentration of at least 100µg/ml. This solution can then be further diluted into other aqueous solutions as needed.
Stability
Lyophilized Oncostatin M remains stable at room temperature for up to 3 weeks; however, for long-term storage, it is recommended to store it desiccated below -18°C. Upon reconstitution, Oncostatin should be stored at 4°C for 2-7 days. For extended storage, freezing below -18°C is recommended, preferably with the addition of a carrier protein like 0.1% HSA or BSA. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 95.0% using the following methods: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis, and (b) SDS-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The ED50, determined by the dose-dependent stimulation of Human TF-1 cells, is less than 2 ng/ml. This corresponds to a Specific Activity of 500,000 IU/mg.
Protein Content
Protein quantification was performed using two independent methods:
1. UV spectroscopy at 280 nm, utilizing an absorbance value of 0.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated using the PC GENE computer analysis program for protein sequences (IntelliGenetics).
2. RP-HPLC analysis, employing a standard solution of Oncostatin as a reference.
Synonyms
OSM, MGC20461.
Source
Escherichia Coli.
Amino Acid Sequence
AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR
ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER
SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP
PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRRHSPH
QALRKGVRRT RPSRKGKRLM TRGQLPR.
ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER
SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP
PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRRHSPH
QALRKGVRRT RPSRKGKRLM TRGQLPR.
Product Science Overview
Structure and Production
Human recombinant Oncostatin-M is typically produced using E. coli expression systems. The protein consists of 196 amino acids and has a molecular mass of approximately 22 kDa . The recombinant form is often purified to a high degree of purity, typically greater than 97%, and is available in both carrier-free and carrier-containing formulations .
Biological Functions
Oncostatin-M plays a crucial role in various physiological processes, including:
Applications
Recombinant Oncostatin-M is widely used in research and therapeutic applications:
- Cell Differentiation: It is used in the differentiation of human pluripotent stem cells into hepatocyte-like cells .
- Cancer Research: Due to its growth-inhibitory effects on certain cancer cells, it is studied for potential therapeutic applications in oncology .
- Inflammatory Diseases: OSM is also investigated for its role in inflammatory diseases and its potential as a therapeutic target .
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