OSM Mouse

Oncostatin M was first identified for its ability to inhibit the growth of A375 melanoma cells and other human tumor cells, while not affecting the growth of normal human fibroblasts . It is primarily produced by activated T cells and macrophages . OSM plays a significant role in immune regulation, fibroblast proliferation, and inhibition of tumor cell proliferation .

Recombinant Mouse Oncostatin M (mOSM) is a laboratory-produced version of the naturally occurring cytokine. It is typically derived from E. coli and has a molecular weight of approximately 21 kDa . The recombinant form is used extensively in research to study its biological activities and potential therapeutic applications.

The biological activity of recombinant mOSM is measured using cell proliferation assays. For instance, it has been shown to induce the proliferation of NIH/3T3 mouse embryonic fibroblast cells . The effective dose (ED50) for this effect is typically in the range of 0.10-0.40 ng/mL .

Recombinant mOSM is used in various research areas, including:

• Cancer Research: Investigating its role in inhibiting tumor cell proliferation.
• Immunology: Studying its effects on immune regulation and cytokine signaling pathways.
• Cell Biology: Understanding its role in cell proliferation and differentiation.

Recombinant mOSM is typically lyophilized and can be reconstituted in sterile PBS. It is stable for up to 12 months when stored at -20 to -70°C in its lyophilized form . After reconstitution, it should be stored under sterile conditions at 2-8°C for up to one month or at -20 to -70°C for up to three months .