OSM Human, 209 a.a
Oncostatin M Human Recombinant (209 a.a.)
Cat. No.
BT25176
Source
Escherichia Coli.
Synonyms
OSM, MGC20461, Oncostatin M.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Oncostatin-M (209 a.a.) Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 209 amino acids and having a molecular mass of 23.9kDa.
The Oncostatin-M (209 a.a.) is purified by proprietary chromatographic techniques.
The Oncostatin-M (209 a.a.) is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Oncostatin M, a cytokine belonging to the same family as leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6, is encoded by a gene that regulates growth and inhibits the proliferation of various tumor cell lines. It influences the production of cytokines like IL-6, G-CSF, and GM-CSF from endothelial cells.
Description
Recombinant Human Oncostatin-M (209 a.a.), produced in E.Coli, is a single, non-glycosylated polypeptide chain composed of 209 amino acids. With a molecular weight of 23.9kDa, this purified protein is obtained through proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Oncostatin-M (209 a.a.) was lyophilized from a concentrated solution (1mg/ml) in 1x PBS pH-7.4.
Solubility
To reconstitute the lyophilized Oncostatin-M (209 a.a.), it is recommended to dissolve it in sterile 18MΩ-cm H2O to a concentration not less than 100µg/ml. This solution can then be further diluted in other aqueous solutions.
Stability
Lyophilized Oncostatin-M (209 a.a.) remains stable at room temperature for up to 3 weeks. However, for long-term storage, it should be kept desiccated below -18°C. Once reconstituted, Oncostatin-M (209 a.a.) should be stored at 4°C for 2-7 days and frozen below -18°C for future use. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 97.0% as determined by (a) RP-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
The ED50, determined by the dose-dependent stimulation of Human TF-1 cells, is less than 2 ng/ml, which translates to a Specific Activity of 500,000 IU/mg.
Synonyms
OSM, MGC20461, Oncostatin M.
Source
Escherichia Coli.
Amino Acid Sequence
AAIGSCSKEYRVLLGQLQKQTDLMQDTSRLLDPYIRIQGLDVPKLREHCRERPG
AFPSEETLRGLGRRGFLQTLNATLGCVLHRLADLEQRLPKAQDLERSGLNIEDL
EKLQMARPNILGLRNNIYCMAQLLDNSDTAEPTKAGRGASQPPTPTPASDAFQ
RKLEGCRFLHGYHRFMHSVGRVFKWGESPNRSRRHSPHQALRKGVRR.
AFPSEETLRGLGRRGFLQTLNATLGCVLHRLADLEQRLPKAQDLERSGLNIEDL
EKLQMARPNILGLRNNIYCMAQLLDNSDTAEPTKAGRGASQPPTPTPASDAFQ
RKLEGCRFLHGYHRFMHSVGRVFKWGESPNRSRRHSPHQALRKGVRR.
Product Science Overview
Structure and Production
The recombinant form of Oncostatin M is produced using Escherichia coli (E. coli) expression systems. This method ensures high yield and purity of the protein. The recombinant protein has a molecular weight of approximately 23.6 kDa . The amino acid sequence of Oncostatin M is as follows:
AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRRHSPH QALRKGVRR
Biological Functions
Oncostatin M is involved in a variety of biological functions:
- Cell Proliferation and Differentiation: OSM can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells, and Kaposi’s sarcoma cells, while inhibiting the growth of some normal and tumor cell lines .
- Cytokine Release: OSM promotes the release of cytokines such as IL-6, GM-CSF, and G-CSF from endothelial cells .
- Neurogenesis and Osteogenesis: It plays a role in the regulation of neurogenesis and osteogenesis, contributing to the development and maintenance of the nervous and skeletal systems .
Therapeutic Potential
Due to its diverse biological activities, Oncostatin M has potential therapeutic applications:
- Cancer Therapy: OSM’s ability to inhibit the growth of certain tumor cell lines makes it a candidate for cancer therapy.
- Inflammatory Diseases: Its role in cytokine release and inflammation suggests potential use in treating inflammatory diseases.
- Bone and Nervous System Disorders: OSM’s involvement in osteogenesis and neurogenesis indicates potential therapeutic applications in bone and nervous system disorders .
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