OSM Human, His

Oncostatin M (OSM) is a glycoprotein belonging to the interleukin-6 (IL-6) family of cytokines . It is encoded by the OSM gene in humans and is known for its pleiotropic functions, meaning it can affect multiple different biological processes . The recombinant form of this protein, tagged with a polyhistidine (His) tag, is produced using recombinant DNA technology, which allows for its expression in host cells such as HEK293 cells .

OSM is involved in various biological processes, including:

• Cell Growth and Differentiation: OSM plays a crucial role in regulating cell growth and differentiation. It has been shown to inhibit the proliferation of certain tumor cell lines while stimulating the proliferation of others, such as AIDS-KS cells .
• Cytokine Production: OSM regulates the production of other cytokines, including IL-6, granulocyte-colony stimulating factor (G-CSF), and granulocyte-macrophage colony-stimulating factor (GM-CSF) from endothelial cells .
• Liver Development and Regeneration: OSM is involved in the maturation of fetal hepatocytes, promoting liver development and regeneration .
• Inflammation and Bone Metabolism: OSM is produced by osteoblasts, bone marrow macrophages, and neutrophils, and it acts as an inflammatory cytokine. It plays a role in bone formation and destruction .

OSM exerts its biological effects by binding to specific cell surface receptors. There are two types of OSM receptors:

• Type I OSM Receptor: This receptor is a heterodimer composed of the leukemia inhibitory factor receptor (LIFR) and the signal transducer gp130 .
• Type II OSM Receptor: This receptor is a heterodimer composed of the OSM receptor (OSMR) and gp130 .

Upon binding to these receptors, OSM activates various intracellular signaling pathways that mediate its diverse biological effects.

The recombinant form of OSM tagged with a His tag is produced by expressing the mature form of human OSM (Met 1-Arg 221) in host cells like HEK293 . The His tag, typically added at the C-terminus, facilitates the purification of the protein using affinity chromatography techniques. The recombinant protein is usually lyophilized from a sterile PBS solution and can be reconstituted for use in various assays .

Recombinant OSM (His tag) is stable for up to twelve months when stored at -20°C to -80°C under sterile conditions . It is recommended to aliquot the protein to avoid repeated freeze-thaw cycles, which can degrade the protein’s activity .

Recombinant OSM (His tag) is used in various research applications, including:

• Cell Proliferation Assays: It is used to measure the proliferation of cells, such as TF-1 human erythroleukemic cells .
• Cytokine Production Studies: It is used to study the regulation of cytokine production in endothelial cells .
• Liver Development Research: It is used to investigate the role of OSM in liver development and regeneration .