MAPK3 Human, His
Description
Product Specs
Product Science Overview
Mitogen-Activated Protein Kinase 3 (MAPK3), also known as Extracellular Signal-Regulated Kinase 1 (ERK1), is a crucial component of the MAP kinase signal transduction pathway. This pathway plays a significant role in regulating various cellular processes, including proliferation, differentiation, and cell cycle progression, in response to extracellular signals .
MAPK3 is a serine/threonine kinase that is activated by upstream kinases. Upon activation, it translocates to the nucleus, where it phosphorylates nuclear targets. This phosphorylation event is essential for the regulation of gene expression and cellular responses to external stimuli .
The human recombinant form of MAPK3, tagged with a His-tag, is often used in research to study its function and interactions. The His-tag facilitates the purification of the protein using affinity chromatography, making it easier to isolate and study in laboratory settings.
MAPK3, along with MAPK1 (ERK2), is a key player in the MAPK/ERK cascade. This cascade is initiated by various extracellular signals, such as growth factors, cytokines, and environmental stresses. The activation of MAPK3 leads to a series of phosphorylation events that ultimately result in the regulation of gene expression .
Some of the critical cellular processes regulated by MAPK3 include:
- Cell Proliferation: MAPK3 promotes cell division and growth by regulating the expression of genes involved in the cell cycle.
- Differentiation: It plays a role in the differentiation of various cell types by modulating the activity of transcription factors.
- Survival and Apoptosis: MAPK3 can influence cell survival and apoptosis, depending on the context and type of extracellular signals received.
Dysregulation of MAPK3 has been associated with various diseases, including cancer, neurological disorders, and inflammatory conditions. For instance, aberrant activation of the MAPK/ERK pathway is commonly observed in many types of cancer, where it contributes to uncontrolled cell proliferation and survival .
The human recombinant MAPK3 with a His-tag is widely used in research to:
- Study Protein-Protein Interactions: Researchers use it to identify and characterize interactions with other proteins involved in the MAPK/ERK pathway.
- Investigate Signaling Pathways: It helps in understanding the detailed mechanisms of the MAPK/ERK signaling cascade.
- Drug Development: MAPK3 is a target for developing therapeutic agents aimed at modulating its activity in diseases where the MAPK/ERK pathway is dysregulated.
