MAPK9 Human
Mitogen-Activated Protein Kinase 9 Human Recombinant
Cat. No.
BT12211
Source
Escherichia Coli.
Synonyms
Mitogen-activated protein kinase 9, MAP kinase 9, MAPK 9, JNK-55, Stress-activated protein kinase 1a, SAPK1a, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, MAPK9, JNK2, PRKM9, SAPK, p54a, JNK2A, JNK2B, JNK2BETA, p54aSAPK, JNK2ALPHA.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
MAPK9 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 406 amino acids (1-382 a.a) and having a molecular mass of 46.6kDa.
MAPK9 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
MAPK9 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Mitogen-activated protein kinase 9 (MAPK9), also known as JNK2, is a member of the MAP kinase family. These kinases play a crucial role in cellular signaling, acting as a central hub for various biochemical signals. They are implicated in a wide array of cellular processes, including cell growth and division (proliferation), specialization of cells (differentiation), regulation of gene expression (transcription regulation), and overall development. MAPK9 specifically targets and regulates transcription factors, thereby controlling the expression of immediate-early genes in response to diverse cellular stimuli. Functioning as an active dimer, MAPK9 can translocate to the nucleus, where it influences transcription through its interactions with transcription factors such as c-Jun, ATF-2, and others. Additionally, MAPK9 has been shown to inhibit the degradation of the tumor suppressor protein p53 by blocking its ubiquitination, thus enhancing p53 stability in unstressed cells.
Description
This product consists of the recombinant human MAPK9 protein, expressed in E. coli and purified to a high degree. The protein is a single, non-glycosylated polypeptide chain that encompasses amino acids 1 to 382 of the MAPK9 sequence. A 24 amino acid His-tag is fused to the N-terminus to facilitate purification. The molecular weight of the purified protein is 46.6 kDa.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The MAPK9 protein is supplied in a solution at a concentration of 1 mg/ml. The solution also contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein such as HSA or BSA to a final concentration of 0.1% is advised. Repeated freezing and thawing of the product should be avoided.
Purity
Analysis by SDS-PAGE demonstrates that the purity of this product is greater than 95%.
Synonyms
Mitogen-activated protein kinase 9, MAP kinase 9, MAPK 9, JNK-55, Stress-activated protein kinase 1a, SAPK1a, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, MAPK9, JNK2, PRKM9, SAPK, p54a, JNK2A, JNK2B, JNK2BETA, p54aSAPK, JNK2ALPHA.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSDSKC DSQFYSVQVA DSTFTVLKRY QQLKPIGSGA QGIVCAAFDT VLGINVAVKK LSRPFQNQTH AKRAYRELVL LKCVNHKNII SLLNVFTPQK TLEEFQDVYL VMELMDANLC QVIHMELDHE RMSYLLYQML CGIKHLHSAG IIHRDLKPSN IVVKSDCTLK ILDFGLARTA CTNFMMTPYV VTRYYRAPEV ILGMGYKENV DIWSVGCIMG ELVKGCVIFQ GTDHIDQWNK VIEQLGTPSA EFMKKLQPTV RNYVENRPKY PGIKFEELFP DWIFPSESER DKIKTSQARD LLSKMLVIDP DKRISVDEAL RHPYITVWYD PAEAEAPPPQ IYDAQLEERE HAIEEWKELI YKEVMDWEER SKNGVVKDQP SAQMQQ.
Product Science Overview
Function and Mechanism
MAPK9 is involved in the integration of multiple biochemical signals. It targets specific transcription factors, mediating immediate-early gene expression in response to various cell stimuli . This kinase is closely related to MAPK8, and both are involved in UV radiation-induced apoptosis, which is thought to be related to the cytochrome c-mediated cell death pathway .
Role in Cellular Processes
MAPK9 plays a significant role in:
- Proliferation and Differentiation: It helps in the regulation of cell growth and differentiation.
- Transcription Regulation: It influences the transcription of genes in response to cellular stimuli.
- Development: It is involved in the development of various tissues and organs.
- Apoptosis: It is crucial in the process of programmed cell death, particularly in response to stress signals like UV radiation .
Interaction with p53
Synthetic Routes and Preparation
The recombinant form of MAPK9 is typically produced using Escherichia coli (E. coli) expression systems. The gene encoding MAPK9 is cloned into an expression vector, which is then introduced into E. coli cells. The bacteria are cultured, and the protein is expressed and subsequently purified using various chromatographic techniques .
Clinical and Research Implications
MAPK9 has been associated with several diseases, including multiple endocrine neoplasia and kidney disease . Its role in apoptosis and cell cycle regulation makes it a significant target for cancer research. Understanding the pathways and mechanisms involving MAPK9 can lead to the development of new therapeutic strategies for various diseases.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
