MAPK3 Human, Active

Mitogen-Activated Protein Kinase 3 Human Recombinant, Active
Cat. No.
BT11997
Source
Escherichia Coli.
Synonyms
Mitogen-activated protein kinase 3, EC 2.7.11.24, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAPK 1, p44-ERK1, ERT2, p44-MAPK, Microtubule-associated protein 2 kinase, ERK1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, MGC20180.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ERK1/MAPK3 Recombinant is a highly active form produced by phosphorylation of the purified ERK1/MAPK3 in vitro with MEK1 is a non-glycosylated polypeptide having a molecular mass of 43.6 kDa.
ERK1/MAPK3 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Extracellular signal-regulated kinases (ERKs), or classical MAP kinases, are signaling proteins found within cells. They play a crucial role in various cellular functions, including cell division (meiosis and mitosis) and specialized functions in mature cells. ERKs are activated by a wide range of stimuli such as growth factors, cytokines, viral infections, and more. They are a key part of the MAPK/ERK pathway, which is activated by signals from outside the cell, ultimately influencing gene expression and other cellular processes. Dysregulation of the ERK pathway, often involving components like Ras, c-Raf, and receptors like HER2, is frequently observed in cancer.
Description
ERK1/MAPK3 Recombinant is a highly active form of the ERK1/MAPK3 protein. It is produced by a process called phosphorylation, which activates the protein. This process is carried out in a controlled laboratory setting using purified ERK1/MAPK3 and another protein called MEK1. This specific ERK1/MAPK3 product is not glycosylated and has a molecular weight of 43.6 kDa. The purification of ERK1/MAPK3 is achieved through specialized chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
ERK1/MAPK3 is supplied in a solution containing 50mM Tris-HCl, 150mM NaCl, 1mM DTT, and 50% glycerol at a pH of 8.5. The concentration of ERK1/MAPK3 in this solution is 0.15mg/ml.
Unit Definition
No protease activity is detectable. The specific activity is greater than 15,000 units per milligram of protein. One unit is defined as the amount of enzyme required to transfer 1 picomole of phosphate to myelin basic protein per minute at 30 degrees Celsius.
Stability

For optimal storage, keep at 4°C if the entire vial will be used within 2-4 weeks. For long-term storage, freeze at -20°C. Repeated freezing and thawing should be avoided.

Purity
The purity of the ERK1/MAPK3 protein is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Mitogen-activated protein kinase 3, EC 2.7.11.24, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAPK 1, p44-ERK1, ERT2, p44-MAPK, Microtubule-associated protein 2 kinase, ERK1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, MGC20180.
Source
Escherichia Coli.

Product Science Overview

Introduction

Mitogen-Activated Protein Kinase 3 (MAPK3), also known as ERK1, is a crucial protein in the MAP kinase signaling pathway. This pathway is essential for various cellular processes, including proliferation, differentiation, and response to external stress signals. MAPK3 is a protein-coding gene and is widely expressed in many tissues.

Structure and Activation

MAPK3 is activated through a cascade of phosphorylation events. The activation process typically begins with the small guanosine triphosphatase (GTPase) Ras, which, upon stimulation by extracellular signals, activates the protein kinases Raf and MEK. MEK, in turn, phosphorylates and activates MAPK3 .

Functions

MAPK3 plays a pivotal role in several cellular functions:

  • Cell Proliferation: It is involved in the regulation of cell division and growth.
  • Differentiation: MAPK3 helps in the specialization of cells into different types.
  • Stress Response: It participates in the cellular response to environmental stressors.
  • Signal Transduction: MAPK3 is a key player in transmitting signals from the cell surface to the nucleus.
Clinical Significance

Mutations or dysregulation of MAPK3 have been associated with various diseases, including Autism Spectrum Disorder and Epilepsy . Understanding the role of MAPK3 in these conditions can help in developing targeted therapies.

Recombinant MAPK3

Human recombinant MAPK3 is produced using recombinant DNA technology, which allows for the expression of the human MAPK3 gene in a host organism, such as bacteria or yeast. This recombinant protein is used in research to study the MAP kinase signaling pathway and its implications in health and disease.

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