Leptin Ovine
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Description
The Leptin is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Leptin ovine recombinant, often referred to as roLeptin, is a recombinant form of leptin derived from sheep. It is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain consisting of 146 amino acids. The molecular mass of leptin ovine recombinant is approximately 16 kDa . The production process involves proprietary chromatographic techniques to ensure high purity and quality.
Leptin exerts its effects by binding to the leptin receptor (LEPR), which is primarily located in the hypothalamus of the brain. Upon binding to its receptor, leptin activates several intracellular signaling pathways that regulate appetite and energy expenditure. The majority of LEPR is localized in intracellular compartments, including the endoplasmic reticulum, trans-Golgi apparatus, and endosomes, with only a minor fraction present at the plasma membrane .
Leptin plays a vital role in various physiological processes, including:
- Regulation of Food Intake: Leptin inhibits appetite by signaling to the brain that the body has sufficient energy stores, thereby reducing food intake.
- Energy Expenditure: Leptin increases energy expenditure by promoting the oxidation of fatty acids and enhancing thermogenesis.
- Reproductive Function: Leptin is involved in the regulation of reproductive function by influencing the hypothalamic-pituitary-gonadal axis.
- Immune Function: Leptin modulates immune responses by affecting the production and activity of various immune cells.
Leptin has significant clinical implications, particularly in the context of obesity and metabolic disorders. Individuals with congenital leptin deficiency or mutations in the LEPR gene exhibit severe early-onset obesity and hyperphagia (excessive eating). Leptin replacement therapy has been shown to be effective in treating these conditions by restoring normal appetite and energy balance .
However, the majority of obese individuals do not have mutations in the leptin or LEPR genes but often exhibit elevated circulating levels of leptin, a condition known as leptin resistance. This condition is characterized by the body’s inability to respond to leptin’s signals, leading to continued overeating and weight gain. Understanding the molecular mechanisms underlying leptin resistance remains a significant challenge in obesity research .
Recombinant leptin, including leptin ovine recombinant, is widely used in research to study its physiological and biochemical effects. It is also utilized in various experimental models to investigate the mechanisms of leptin signaling and resistance. Additionally, leptin has potential therapeutic applications in treating metabolic disorders, obesity, and related conditions.
