Leptin Salamander

Leptin Salamander Recombinant is a recombinant form of leptin derived from salamanders. It is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain containing 146 amino acids, with an additional alanine at the N-terminus. The molecular mass of this recombinant protein is approximately 16 kDa .

The production of Leptin Salamander Recombinant involves the expression of the leptin gene in E. coli. The recombinant protein is then purified using proprietary chromatographic techniques to ensure high purity. The final product is a sterile, lyophilized (freeze-dried) white powder .

• Formulation: The lyophilized leptin is typically reconstituted in a sterile solution of 0.4% NaHCO3 at pH 9, at a concentration of not less than 100 µg/ml. This solution can then be further diluted for various applications .
• Stability: The lyophilized form is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage. Once reconstituted, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use. It is recommended to add a carrier protein (0.1% HSA or BSA) to prevent freeze-thaw cycles .
• Purity: The purity of Leptin Salamander Recombinant is greater than 99.0%, as determined by gel-filtration and SDS-PAGE analysis .

Leptin Salamander Recombinant is biologically active and can induce the proliferation of BAF/3 cells stably transfected with the long form of the human leptin receptor. Its activity in this assay is comparable to that of mouse leptin .

Leptin Salamander Recombinant is primarily used for laboratory research purposes. It is not intended for use as a drug, agricultural or pesticidal product, food additive, or household chemical .

As with all recombinant proteins, proper safety and handling procedures should be followed. The product should be used in a controlled laboratory environment and handled by trained personnel .