Leptin Rat
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Description
The Leptin is purified by proprietary chromatographic techniques.
Product Specs
(a) SEC-HPLC analysis.
(b) SDS-PAGE analysis.
1. UV spectroscopy at 280 nm, utilizing an absorbance value of 0.201 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is derived from the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. RP-HPLC analysis, employing a calibrated solution of Leptin rat as a reference standard.
Product Science Overview
Leptin was first identified as the protein product of the mouse obese (ob) gene. Mice with mutations in the obese gene that block the synthesis of leptin are found to be obese and diabetic, with reduced activity, metabolism, and body temperature . The discovery of leptin has significantly advanced our understanding of obesity and metabolic disorders.
Recombinant leptin refers to leptin that is produced through recombinant DNA technology. This involves inserting the gene that encodes leptin into a host organism, such as Escherichia coli (E. coli), to produce the hormone in large quantities. Recombinant rat leptin shares approximately 96% and 82% sequence identity with the mouse and human proteins, respectively .
The preparation of recombinant leptin typically involves the following steps:
- Gene Cloning: The gene encoding leptin is cloned into an expression vector.
- Transformation: The expression vector is introduced into a host organism, such as E. coli.
- Expression: The host organism is cultured under conditions that promote the expression of leptin.
- Purification: The leptin protein is purified from the host organism using techniques such as affinity chromatography.
Leptin regulates various physiological processes, including:
- Food Intake and Energy Expenditure: Leptin signals the brain to reduce appetite and increase energy expenditure.
- Insulin Sensitivity: Leptin enhances insulin sensitivity, which is crucial for glucose metabolism.
- Fertility: Leptin plays a role in reproductive function by regulating the hypothalamic-pituitary-gonadal axis.
- Immune System: Leptin has proinflammatory effects and regulates both innate and adaptive immune responses.
- Bone Metabolism: Leptin influences bone mass and density .
Recombinant rat leptin is widely used in research to study obesity, diabetes, and related metabolic disorders. It is also used to investigate the role of leptin in various physiological processes and disease states. For example, leptin knockout rats, which lack the leptin gene, are used as models to study the effects of leptin deficiency .
