Leptin Rabbit

Leptin (Rabbit Recombinant) is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain containing 146 amino acids. The molecular mass of this recombinant leptin is approximately 16 kDa . The protein is purified using proprietary chromatographic techniques to ensure high purity and quality.

Recombinant leptin is biologically active and has been shown to induce the proliferation of BAF/3 cells stably transfected with the long form of the human leptin receptor . This activity is crucial for studying leptin’s role in various physiological processes, including energy homeostasis, immune response, and metabolic regulation.

Lyophilized leptin is stable at room temperature for up to three weeks. However, for long-term storage, it should be kept desiccated below -18°C. Upon reconstitution, leptin should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use. To prevent degradation, it is recommended to add a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) and avoid freeze-thaw cycles .

Recombinant leptin is widely used in laboratory research to study its effects on various biological processes. It is particularly valuable in research related to obesity, diabetes, and metabolic disorders. Leptin’s role in regulating food intake and energy expenditure makes it a critical target for understanding and potentially treating these conditions.