Leptin Ovine, MTS
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Description
The Leptin is purified by proprietary chromatographic techniques. The membrane translocating sequence Tag is composed of 10 amino acids Val-Leu-Leu-Pro-Val-Leu-Leu-Ala-Ala-Pro located at the N-terminus.
Product Specs
Product Science Overview
Leptin is a protein hormone predominantly produced by adipocytes (fat cells) and plays a crucial role in regulating energy balance by inhibiting hunger . It is encoded by the LEP gene and is involved in various physiological processes, including metabolism, endocrine regulation, and immune function . Leptin’s primary function is to signal the brain about the body’s energy reserves, thereby regulating appetite and energy expenditure .
Ovine leptin refers to leptin derived from sheep (Ovis aries), a species within the genus Ovis . Sheep are domesticated ruminant mammals known for their wool, meat, and milk production . Ovine leptin shares similar functions with leptin from other species, contributing to the regulation of energy balance and various metabolic processes.
Recombinant proteins are proteins that are genetically engineered by inserting the gene encoding the protein of interest into a host cell, such as bacteria, yeast, or mammalian cells . This technology allows for the production of large quantities of specific proteins for research, therapeutic, and industrial applications . Recombinant proteins can have the same amino acid sequence as the naturally occurring protein or be modified for improved properties .
Leptin Ovine Recombinant is a form of leptin produced using recombinant DNA technology. It involves inserting the ovine leptin gene into a host cell, typically E. coli, to produce the protein in large quantities . This recombinant leptin is a single, non-glycosylated polypeptide chain containing 157 amino acids and has a molecular mass of approximately 17.5 kDa . The recombinant leptin is purified using proprietary chromatographic techniques to ensure its purity and functionality .
The MTS (Mitochondrial Targeting Sequence) tag is a short peptide sequence that directs the transport of the tagged protein to the mitochondria . MTS tags are typically located at the N-terminus of the protein and are recognized by the mitochondrial import machinery, facilitating the protein’s import into the mitochondria . In the context of Leptin Ovine Recombinant, the MTS tag ensures that the leptin protein is correctly localized within the mitochondria, where it can perform its intended functions.
Leptin Ovine Recombinant with an MTS tag has several applications in scientific research and biotechnology. It is used to study the role of leptin in energy regulation, metabolism, and various physiological processes. Additionally, it can be employed in the development of therapeutic interventions for metabolic disorders, such as obesity and diabetes, by providing insights into leptin’s mechanisms of action and potential therapeutic targets.
