Leptin qA Ovine
(a) Gel filtration analysis.
(b) Analysis by SDS-PAGE.
Description
Leptin Antagonist Quadruple Mutant Ovine Recombinant was purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Leptin is a protein hormone predominantly produced by adipocytes (fat cells) and plays a crucial role in regulating energy balance by inhibiting hunger . It is often referred to as the “satiety hormone” or “starvation hormone” because it helps to regulate appetite and energy expenditure . Leptin levels are directly proportional to the amount of body fat, and it signals the brain about the energy status of the body .
Leptin antagonists are modified forms of leptin that inhibit its action. These antagonists are valuable in research and potential therapeutic applications, particularly in conditions where leptin’s effects need to be blocked, such as certain autoimmune diseases, cancer, and obesity . Leptin antagonists work by binding to leptin receptors without activating them, thereby preventing the natural leptin from exerting its effects .
The term “quadruple mutant” refers to a leptin molecule that has undergone four specific mutations. In the case of the Leptin Antagonist Quadruple Mutant, the mutations are L39A, D40A, F41A, and I42A . These mutations are designed to enhance the antagonist properties of leptin, making it more effective in blocking leptin receptors .
“Ovine” refers to sheep, and “recombinant” indicates that the protein is produced using recombinant DNA technology. Recombinant proteins are generated by inserting the gene encoding the protein into a host organism, such as bacteria or yeast, which then produces the protein . Ovine recombinant leptin is leptin derived from sheep and produced using this technology .
The Leptin Antagonist Quadruple Mutant Ovine Recombinant is a specific form of leptin antagonist that has been engineered with four mutations and produced using recombinant DNA technology in sheep . This protein is a single non-glycosylated polypeptide chain containing 146 amino acids, with an additional alanine at the N-terminus, and has a molecular mass of approximately 16 kDa .
The Leptin Antagonist Quadruple Mutant Ovine Recombinant is significant in both research and potential therapeutic applications. It is used to study the role of leptin in various physiological processes and diseases. By blocking leptin’s action, researchers can better understand its functions and develop new treatments for conditions such as obesity, autoimmune diseases, and cancer .
