Leptin qA Human

Leptin is a protein hormone primarily produced by adipocytes (fat cells) and plays a crucial role in regulating body weight, metabolism, and reproductive functions. It is encoded by the obese (ob) gene and has a molecular weight of approximately 16 kDa . Leptin exerts its effects by binding to leptin receptors, which are highly expressed in the hypothalamus, T lymphocytes, and vascular endothelial cells .

The Leptin Antagonist Quadruple Mutant (Human Recombinant) is a modified form of human leptin designed to inhibit the biological effects of leptin. This mutant protein is a single polypeptide chain containing 146 amino acids, with an additional alanine at the N-terminus . The specific mutations introduced in this protein are L39A, D40A, F41A, and I42A . These mutations significantly reduce the binding affinity of leptin to its receptor, thereby acting as an antagonist.

The Leptin Antagonist Quadruple Mutant is produced using recombinant DNA technology in an E. coli expression system . The protein is then purified through proprietary chromatographic techniques to achieve a purity greater than 98%, as determined by gel filtration analysis and SDS-PAGE . The final product is typically formulated as a white lyophilized (freeze-dried) powder and can be reconstituted for use in various experimental settings .

The primary function of the Leptin Antagonist Quadruple Mutant is to inhibit leptin-induced biological activities. It has been shown to effectively inhibit the proliferation of BAF/3 cells stably transfected with the long form of the human leptin receptor . Additionally, it can inhibit various leptin effects in several in vitro bioassays, making it a valuable tool for studying leptin signaling pathways and their physiological implications .

The Leptin Antagonist Quadruple Mutant is widely used in research to investigate the role of leptin in body weight regulation, metabolism, and reproductive functions. It is particularly useful in studies aiming to understand leptin resistance, a condition often associated with obesity and metabolic disorders . By blocking leptin signaling, researchers can gain insights into the mechanisms underlying leptin resistance and develop potential therapeutic strategies for related conditions.

The lyophilized Leptin Antagonist Quadruple Mutant is stable at room temperature for several weeks but should be stored desiccated below -18°C for long-term storage . Upon reconstitution, the protein can be stored at 4°C or even room temperature for several weeks, making it suitable for long-term infusion studies using osmotic pumps . To prevent degradation, it is recommended to avoid freeze-thaw cycles and add a carrier protein (e.g., 0.1% HSA or BSA) at lower concentrations .