Leptin tA Ovine, PEG
(a) Gel filtration analysis.
(b) Analysis by SDS-PAGE.
Description
Pegylated Leptin Antagonist Triple Mutant Ovine Recombinant is a single non-glycosilated polypeptide chain containing 146 aa and an additional Ala at N-terminus and having a molecular mass of ~ 35.6 kDa, Leptin was mutated, resulting in L39A/D40A/F41A mutant. However due to enlarged hydrodymanic volume it runs on the SGS-Page as 48 kDa protein and in gel-filtration on Superdex 200 as over 200kDa protein. Leptin Antagonist Triple Mutant Ovine Recombinant Mono-Pegylated with 20kDa PEG and was purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Leptin is a hormone predominantly made by adipose cells and enterocytes in the small intestine that helps to regulate energy balance by inhibiting hunger. It is often referred to as the “satiety hormone” or “fat hormone.” Leptin’s primary target is the hypothalamus in the brain where it inhibits appetite by counteracting the effects of neuropeptide Y, a potent feeding stimulant secreted by cells in the gut and in the hypothalamus.
Leptin antagonists are molecules designed to inhibit the action of leptin. These antagonists are particularly useful in research settings to study the physiological and pathological roles of leptin. They can help in understanding leptin resistance, a condition often associated with obesity where the body does not respond to leptin signals, leading to uncontrolled food intake and weight gain.
The Leptin Antagonist Triple Mutant Ovine Recombinant is a specially engineered form of leptin. It is derived from ovine (sheep) leptin and has been mutated at three specific sites: L39A, D40A, and F41A. These mutations are designed to disrupt the binding of leptin to its receptor, effectively turning it into an antagonist. This form of leptin is produced recombinantly, meaning it is generated through genetic engineering techniques in a laboratory setting.
Pegylation refers to the process of attaching polyethylene glycol (PEG) chains to another molecule, in this case, the leptin antagonist. Pegylation is a common technique used to improve the pharmacokinetics of therapeutic proteins. It can increase the molecule’s stability, solubility, and half-life in the bloodstream, making it more effective for research and potential therapeutic applications.
The Pegylated Leptin Antagonist Triple Mutant Ovine Recombinant is a single non-glycosylated polypeptide chain containing 146 amino acids, with an additional alanine at the N-terminus. It has a molecular mass of approximately 35.6 kDa . The pegylation process enhances its stability and prolongs its activity in biological systems.
This leptin antagonist is primarily used in research to study the role of leptin in various physiological processes, including appetite regulation, energy balance, and metabolic disorders. It can also be used to investigate leptin resistance and its implications in obesity and related conditions.
