KIR2DL3 Human
Killer Cell Immunoglobulin-Like Receptor, 2 Domains Long Cytoplasmic Tail 3 Human Recombinant
Cat. No.
BT26307
Source
Escherichia Coli.
Synonyms
Killer cell immunoglobulin-like receptor 2DL3, MHC class I NK cell receptor, Natural killer-associated transcript 2, NKAT-2, NKAT2a, NKAT2b, p58 natural killer cell receptor clone CL-6, p58 NK receptor, p58.2 MHC class-I-specific NK receptor, Killer inhibitory receptor cl 2-3, KIR-023GB, CD158 antigen-like family member B2, CD158b2 antigen, KIR2DL3, CD158B2, KIRCL23, NKAT2, p58, NKAT, GL183, CD158b, KIR-K7b, KIR-K7c, MGC129943.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
Recombinant KIR2DL3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing amino acids 23-223 and having a molecular mass of 22.2kDa.
The KIR2DL3 is purified by proprietary chromatographic techniques.
The KIR2DL3 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Killer-cell immunoglobulin-like receptors (KIRs) are proteins found on Natural Killer (NK) cells, which are part of the immune system. KIRs regulate NK cell activity by interacting with MHC class I molecules present on all cells. This interaction helps NK cells identify infected or cancerous cells with low MHC class I levels. Most KIRs are inhibitory, meaning they suppress NK cell activity upon binding to MHC. A few KIRs can activate NK cells. KIR genes are located on chromosome 19q13.4 and exhibit high polymorphism, resulting in diverse KIR gene profiles among individuals. KIR proteins are classified by their extracellular immunoglobulin domains (2D or 3D) and cytoplasmic tail length (long (L) or short (S)). KIRs with long cytoplasmic tails contain an ITIM motif that inhibits NK cell activity upon ligand binding. In contrast, KIRs with short cytoplasmic tails lack the ITIM motif and instead activate NK cells through the TYRO protein tyrosine kinase binding protein. KIR2DL3 is an inhibitory KIR that recognizes MHC class I molecules (HLA-Cw1, -Cw3, -Cw7, and Cw8) and suppresses NK cell activity to prevent cell lysis.
Description
Recombinant KIR2DL3, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising amino acids 23-223. It has a molecular weight of 22.2 kDa. KIR2DL3 is purified using specialized chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The protein solution (1mg/ml) is prepared in 25mM Tris-HCl buffer at pH 7.5.
Stability
For short-term storage (up to 4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Repeated freezing and thawing should be avoided.
Purity
The purity is determined to be greater than 95.0% by SDS-PAGE analysis.
Synonyms
Killer cell immunoglobulin-like receptor 2DL3, MHC class I NK cell receptor, Natural killer-associated transcript 2, NKAT-2, NKAT2a, NKAT2b, p58 natural killer cell receptor clone CL-6, p58 NK receptor, p58.2 MHC class-I-specific NK receptor, Killer inhibitory receptor cl 2-3, KIR-023GB, CD158 antigen-like family member B2, CD158b2 antigen, KIR2DL3, CD158B2, KIRCL23, NKAT2, p58, NKAT, GL183, CD158b, KIR-K7b, KIR-K7c, MGC129943.
Source
Escherichia Coli.
Amino Acid Sequence
MEGVHRKPSL LAHPGPLVKS EETVILQCWS DVRFQHFLLH REGKFKDTLH LIGEHHDGIS KANFSIGPMM QDLAGTYRCY GSVTHSPYQL SAPSDPLDIV ITGLYEKPSL SAQPGPTVLA GESVTLSCSS RSSYDMYHLS REGEAHERRF SAGPKVNGTF QADFPLGPAT HGGTYRCFGS FRDSPYEWSN SSDPLLVSVT GN.
Product Science Overview
Structure and Function
KIR2DL3 is characterized by:
- Two extracellular C2-type immunoglobulin-like domains: These domains are responsible for recognizing specific molecules on the surface of target cells.
- A transmembrane domain: This region anchors the receptor to the cell membrane.
- A long cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs): These motifs are crucial for transmitting inhibitory signals to the NK cell, preventing it from attacking normal, healthy cells .
Genetic and Molecular Background
The KIR2DL3 gene is located on chromosome 19q13.4 . It is part of a gene family that includes several other KIR genes, each with varying specificities and functions. The expression of KIR2DL3 and its interaction with human leukocyte antigen (HLA) molecules are essential for the regulation of NK cell activity. Specifically, KIR2DL3 interacts with HLA-C1 alleles, which helps in distinguishing between healthy cells and potential threats .
Clinical Significance
KIR2DL3 has been associated with various clinical conditions, including:
- Graft-Versus-Host Disease (GVHD): The presence of certain KIR and HLA combinations can influence the outcome of hematopoietic stem cell transplantation .
- Prostate Cancer: Variations in KIR2DL3 expression have been linked to the progression of prostate cancer .
- HIV Resistance: Studies have shown that certain KIR2DL3 genotypes may confer resistance to HIV infection in exposed individuals .
Research and Applications
The recombinant form of KIR2DL3 is used in various research applications to study its role in immune regulation and its potential therapeutic implications. Understanding the interactions between KIR2DL3 and HLA molecules can lead to the development of new treatments for immune-related diseases and conditions.
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