KIR3DL1 Human

Killer Cell Immunoglobulin-Like Receptor, 3 Domains Long Cytoplasmic Tail 1 Human Recombinant
Cat. No.
BT26569
Source
Escherichia Coli.
Synonyms
Killer cell immunoglobulin-like receptor 3DL1, MHC class I NK cell receptor, Natural killer-associated transcript 3, NKAT-3, p70 natural killer cell receptor clones CL-2/CL-11, HLA-BW4-specific inhibitory NK cell receptor, CD158 antigen-like family member E, CD158e antigen, KIR3DL1, CD158E, NKAT3, NKB1, KIR, NKB1B, CD158E1, MGC119726, MGC119728, MGC126589, MGC126591.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Recombinant KIR3DL1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15 kDa.
The KIR3DL1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Killer-cell immunoglobulin-like receptors (KIRs) are a family of proteins found on the surface of natural killer (NK) cells, which are part of the immune system. These receptors help control the NK cells' ability to eliminate other cells. KIRs do this by interacting with molecules called MHC class I, which are present on most cell types. This interaction enables KIRs to detect infected or cancerous cells that have abnormally low levels of MHC class I. Most KIRs are inhibitory, meaning they prevent NK cells from killing when they interact with MHC class I. However, a few KIRs can activate NK cells. KIR genes are located on chromosome 19q13.4 within a region called the leukocyte receptor complex (LRC). KIR genes are highly diverse, meaning individuals have different sets of KIR genes and, therefore, different KIR proteins. KIR proteins are categorized based on their structure, specifically the number of extracellular immunoglobulin domains (2D or 3D) and the length of their intracellular tail (long (L) or short (S)). KIRs with long tails inhibit NK cell activity through a motif called ITIM, while those with short tails activate NK cells by interacting with a protein called TYRO. KIR3DL1, also known as NKB1, nkat3, or p70KIR, is an inhibitory KIR that recognizes a specific region on HLA-B molecules called the Bw4 determinant. This interaction triggers the ITIMs in KIR3DL1's tail to suppress NK cell activity. This product consists of the cytoplasmic tail of KIR3DL1 (amino acids 361-444) with a His-tag, which was initially produced in E. coli as insoluble aggregates (inclusion bodies).
Description
Recombinant KIR3DL1, produced in E. coli, is a single polypeptide chain that is not glycosylated. It contains 132 amino acids and has a molecular weight of 15 kDa. The protein has been purified using proprietary chromatographic methods.
Physical Appearance
A clear and colorless solution that has been sterilized by filtration.
Formulation
The protein solution has a concentration of 1 mg/ml and is formulated in a buffer containing 25mM Tris-HCl (pH 7.5) and 100mM NaCl.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For longer periods, it should be stored frozen at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for extended storage. Avoid repeatedly freezing and thawing the product.
Purity
The purity of the protein is greater than 95%, as determined by RP-HPLC and SDS-PAGE analysis.
Synonyms
Killer cell immunoglobulin-like receptor 3DL1, MHC class I NK cell receptor, Natural killer-associated transcript 3, NKAT-3, p70 natural killer cell receptor clones CL-2/CL-11, HLA-BW4-specific inhibitory NK cell receptor, CD158 antigen-like family member E, CD158e antigen, KIR3DL1, CD158E, NKAT3, NKB1, KIR, NKB1B, CD158E1, MGC119726, MGC119728, MGC126589, MGC126591.
Source
Escherichia Coli.
Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSTSGT IDKLDIEFHLWCSNKKNAAV MDQEPAGNRT ANSEDSDEQD PEEVTYAQLD HCVFTQRKIT RPSQRPKTPP TDTILYTELP NAKPRSKVVS CP.

Product Science Overview

Gene and Protein Structure

The KIR3DL1 gene is located on chromosome 19q13.4 within the leukocyte receptor complex (LRC), a region known for its high density of immune-related genes . The gene encodes a type I transmembrane protein consisting of 444 amino acids. The protein structure includes:

  • A 21-amino acid signal peptide
  • An extracellular region with three C2-type immunoglobulin-like domains
  • Three potential N-linked glycosylation sites
  • A 20-amino acid hydrophobic transmembrane region
  • An 84-amino acid cytoplasmic domain
Function and Mechanism

KIR3DL1 is an inhibitory receptor that interacts with class I major histocompatibility complex (MHC) molecules, specifically HLA-B molecules . Upon binding to its ligand, KIR3DL1 transduces inhibitory signals through immune tyrosine-based inhibitory motifs (ITIMs) present in its long cytoplasmic tail. This interaction helps regulate the activity of NK cells, preventing them from attacking normal, healthy cells .

Polymorphism and Variants

KIR3DL1 is highly polymorphic, with at least 73 known variants. These variants contribute to the diversity of the immune response among individuals. The gene is also closely related to KIR3DS1, an activating receptor with a short cytoplasmic tail. Both genes are sometimes considered alleles of the same gene due to their high sequence homology .

Clinical Significance

The polymorphic nature of KIR3DL1 has been associated with various diseases and conditions. For example, certain variants of KIR3DL1 are linked to a reduced risk of developing graft-versus-host disease (GVHD) following bone marrow transplantation . Additionally, the interaction between KIR3DL1 and its ligands has implications in the context of viral infections, such as HIV, where specific KIR3DL1-HLA combinations can influence the progression of the disease .

Research and Applications

Recombinant forms of KIR3DL1 are used in research to study its structure, function, and interactions with other molecules. These studies provide insights into the mechanisms of immune regulation and potential therapeutic targets for modulating immune responses in various diseases .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

KIR2DL1 Human
Killer Cell Immunoglobulin-Like Receptor, 2 Domains Long Cytoplasmic Tail 1 Human Recombinant
Cat. No.
BT26205
Source
Escherichia Coli.
KIR2DL3 Human
Killer Cell Immunoglobulin-Like Receptor, 2 Domains Long Cytoplasmic Tail 3 Human Recombinant
Cat. No.
BT26307
Source
Escherichia Coli.
KIR2DS4 Human
Killer Cell Immunoglobulin-Like Receptor, 2 Domains Short Cytoplasmic Tail, 4 Recombinant Human
Cat. No.
BT26479
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.