KGF 2 Human

KGF-2 is a single, non-glycosylated polypeptide chain consisting of 170 amino acids, with a molecular weight of approximately 19.3 kDa . It shares 57% sequence homology with KGF-1 (FGF-7) and binds to the same receptor . The protein is highly related to KGF-1 and exhibits mitogenic activity for keratinizing epidermal cells but has minimal activity for fibroblasts .

The biological activity of KGF-2 is significant in various cellular processes. It has been shown to stimulate the proliferation of keratinocytes, which are the predominant cell type in the epidermis. This makes KGF-2 a critical factor in wound healing and tissue repair . The ED50, or the effective dose for 50% of the population, is less than 0.5 ng/mL, corresponding to a specific activity of more than 2 × 10^6 units/mg .

Recombinant human KGF-2 is typically produced in Escherichia coli (E. coli) and purified through sequential chromatography . The production process involves cloning the gene encoding human KGF-2 into an expression vector, which is then transformed into E. coli for large-scale production . The protein is then purified to achieve a purity greater than 95%, as determined by SDS-PAGE and RP-HPLC .

KGF-2 is usually lyophilized from a solution containing 1 mg/mL recombinant KGF-2, 5 mM sodium phosphate (pH 7.4), and 80 mM NaCl . The lyophilized protein is stable at room temperature for up to three weeks but should be stored at -20°C for long-term storage. Upon reconstitution, KGF-2 can be stored at 4°C for 2-7 days .

KGF-2 has a wide range of applications in research and medicine. It is used in studies related to epithelial morphogenesis, repair, and cytoprotection . Its role in wound healing makes it a valuable tool in regenerative medicine and tissue engineering . Additionally, KGF-2 is being explored for its potential in treating various skin conditions and injuries.